NDLI: A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure.

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A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure.

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A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yenofsky, R. L. Fine, M. Pellow, J. W.
Description	Author Affiliation: Yenofsky RL ( Phytogen, Pasadena, CA 91105.);
Abstract	The neo (neomycin-resistance) gene of transposon Tn5 encodes the enzyme neomycin phosphotransferase II (EC 2.7.1.95), which confers resistance to various aminoglycoside antibiotics, including kanamycin and G418. The gene is widely used as a selectable marker in the transformation of organisms as diverse as bacteria, yeast, plants, and animals. We found a mutation that involves a glutamic to aspartic acid conversion at residue 182 in the protein encoded by the chimeric neomycin phosphotransferase II genes of several commonly used transformation vectors. The mutation substantially reduces phosphotransferase activity but does not appear to affect the stability of the neomycin phosphotransferase II mRNA or protein. Plants and bacteria transformed with the mutant gene are less resistant to antibiotics than those transformed with the normal gene. A simple restriction endonuclease digestion distinguishes between the mutant and the normal gene.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	87
Language	English
Publisher	National Academy of Sciences
Publisher Date	1990-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Anti-Bacterial Agents Pharmacology Escherichia Coli Genetics Genes, Bacterial Mutation Phosphotransferases Cells, Cultured Chimera Drug Resistance, Microbial Drug Effects Growth & Development Genetic Vectors Kanamycin Kinase Plants, Toxic Plasmids Promoter Regions, Genetic Terminator Regions, Genetic Tobacco Enzymology Transformation, Genetic Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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