NDLI: High-frequency T-DNA-mediated gene tagging in plants.

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Localization of virus-specific and group-specific epitopes of plant potyviruses by systematic immunochemical analysis of overlapping peptide fragments.

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Direct observations of ligand dynamics in hemoglobin by subpicosecond infrared spectroscopy.

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High-frequency T-DNA-mediated gene tagging in plants.

Inhibition of Ca2+/calmodulin-dependent protein kinase II by arachidonic acid and its metabolites.

Subunits of purified calcium channels: a 212-kDa form of alpha 1 and partial amino acid sequence of a phosphorylation site of an independent beta subunit.

Crystal structure of the Glu-239----Gln mutant of aspartate carbamoyltransferase at 3.1-A resolution: an intermediate quaternary structure.

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Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines.

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Functional comparison of the Rev trans-activators encoded by different primate immunodeficiency virus species.

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Escherichia coli carbamoyl-phosphate synthetase: domains of glutaminase and synthetase subunit interaction.

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Homology analysis of the protein sequences of fatty acid synthases from chicken liver, rat mammary gland, and yeast.

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Isolation of mouse CD44 cDNA: structural features are distinct from the primate cDNA.

Selective loss of cholinergic neurons in the ventral striatum of patients with Alzheimer disease.

Olfactory marker protein gene: its structure and olfactory neuron-specific expression in transgenic mice.

One-way analysis of variance with unequal variances.

Molecular cloning and sequence analysis of 3-hydroxy-3-methylglutaryl-coenzyme A reductase from the human parasite Schistosoma mansoni.

Structural mimicry of adenosine by the antitumor agents 4-methoxy- and 4-amino-8-(beta-D-ribofuranosylamino)pyrimido[5,4-d]pyrimidine as viewed by a molecular modeling method.

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Hydrophobic effect in protein folding and other noncovalent processes involving proteins.

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Expression in transgenic plants of a viral gene product that mediates insect transmission of potyviruses.

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Role of platelets in smooth muscle cell proliferation and migration after vascular injury in rat carotid artery.

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Specific binding of angiogenin to calf pulmonary artery endothelial cells.

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Inhibition of the replication of hepatitis B virus by the carbocyclic analogue of 2'-deoxyguanosine.

Nerve growth factor mRNA and protein increase in hypothalamus in a mouse model of aggression.

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Specific photoaffinity labeling of two plasma membrane polypeptides with an azido auxin.

Phytochrome induces changes in the immunodetectable level of a wall peroxidase that precede growth changes in maize seedlings.

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High-frequency T-DNA-mediated gene tagging in plants.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Körber, H. Redei, G. P. Koncz, C. Mayerhofer, R. Koncz-kalman, Z. Martini, N. Schell, J.
Description	Author Affiliation: Koncz C ( Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.);
Abstract	An insertion element [transferred DNA (T-DNA)], transferred by soil agrobacteria into the nuclear genome of plants, was used for induction of gene fusions in Arabidopsis thaliana, Nicotiana tabacum, and Nicotiana plumbaginifolia. A promoterless aph(3')II (aminoglycoside phosphotransferase II) reporter gene was linked to the right end of the T-DNA and transformed into plants along with a plasmid replicon and a selectable hygromycin-resistance gene. Transcriptional and translational reporter gene fusions were identified by screening for APH(3')II enzyme activity in diverse tissues of transgenic plants. The frequency of gene fusions, estimated by determination of the copy number of T-DNA insertions, showed that on average 30% of T-DNA inserts induced gene fusions in Arabidopsis and Nicotiana. Gene fusions were rescued from plants by transformation of the T-DNA-linked plasmid and flanking plant DNA into Escherichia coli. By dissection of gene fusions and construction of chimeric genes, callus- and root-specific promoters were identified that showed an altered tissue specificity in the presence of a 3'-downstream-located 35S promoter. Transcript mapping of a gene fusion and expression of a non-frame transcriptional fusion of bacterial luciferase luxA and luxB genes demonstrated that dicistronic transcripts are translated in tobacco.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	21
Volume Number	86
Language	English
Publisher	National Academy of Sciences
Publisher Date	1989-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cloning, Molecular DNA, Bacterial Genetics Plants Plasmids Rhizobium DNA Transposable Elements Genes, Bacterial Genetic Vectors Luciferases Molecular Sequence Data Plants, Toxic Promoter Regions, Genetic Tobacco Transcription, Genetic Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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