NDLI: Cloning and cDNA sequence of a bovine submaxillary gland mucin-like protein containing two distinct domains.

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Cloning and cDNA sequence of a bovine submaxillary gland mucin-like protein containing two distinct domains.

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Cloning and cDNA sequence of a bovine submaxillary gland mucin-like protein containing two distinct domains.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Woitach, J. T. Davidson, E. A. Bhavanandan, V. P. Bhargava, A. K.
Description	Author Affiliation: Bhargava AK ( Department of Biological Chemistry, Pennsylvania State University, Hershey, PA 17033.);
Abstract	A lambda gt11 cDNA library prepared from bovine submaxillary gland mRNA was screened with polyclonal anti-apo-bovine submaxillary mucin antibodies with the aim of obtaining the deduced amino acid sequence of the mucin core protein. One of the positive clones had a 1.8 kilobase (kb) cDNA insert and coded for an incomplete protein. A 2.0-kb cDNA clone was isolated by rescreening the library with the 1.8-kb cDNA. Nucleotide sequencing of the full-length 2.0-kb cDNA revealed an open reading frame that coded for a 563-amino acid protein. A striking feature of the cloned protein is the skewed distribution of the amino acids, most notably that of the hydroxy amino acids and cysteine. The amino-terminal domain of 339 residues is very rich in threonine, serine, and glycine and poor in cysteine, aspartic acid, tyrosine, phenylalanine, and tryptophan. In contrast, the carboxyl-terminal domain of 224 residues is rich in cysteine, aspartic acid, tyrosine, lysine, and asparagine and relatively poor in threonine, serine, and glycine. A search of the protein data bank for homologies to the deduced amino acid sequence revealed statistically significant matches to several proteins, including the porcine submaxillary apomucin fragment. The cysteine-rich domain by itself was not statistically homologous with any of the registered polypeptide sequences. RNA blot analysis using DNA probes corresponding to the mucin-like and cysteine-rich regions detected a nearly identical pattern of transcripts, demonstrating that the characterized clones are not artifacts of cDNA library construction. The blots also showed the presence of polydisperse transcripts in bovine submaxillary gland but no detectable hybridization signals in liver or brain RNA.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	17
Volume Number	87
Language	English
Publisher	National Academy of Sciences
Publisher Date	1990-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	DNA Genetics Mucins Submandibular Gland Metabolism Amino Acid Sequence Animals Apoproteins Brain Cloning, Molecular Cysteine Isolation & Purification Escherichia Coli Gene Library Liver Molecular Sequence Data Oligonucleotide Probes RNA, Messenger Restriction Mapping Sequence Homology, Nucleic Acid Comparative Study Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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