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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Rodrigues, N. R. Bodmer, W. F. Rowan, A. Smith, M. E. Kerr, I. B. Gannon, J. V. Lane, D. P. |
| Description | Author Affiliation: Rodrigues NR ( Director's Laboratory, Imperial Cancer Research Fund, Lincolns Inn Fields, London, United Kingdom.); |
| Abstract | Immunohistological staining of primary colorectal carcinomas with antibodies specific to p53 demonstrated gross overexpression of the protein in approximately 50% of the malignant tumors examined. Benign adenomas were all negative for p53 overexpression. To determine the molecular basis for this overexpression we examined p53 protein expression in 10 colorectal cancer cell lines. Six of the cell lines expressed high levels of p53 in ELISA, cell-staining, and immunoprecipitation studies. Direct sequencing and chemical-mismatch-cleavage analysis of p53 cDNA by using the polymerase chain reaction in these cell lines showed that all cell lines that expressed high levels of p53 were synthesizing mRNAs that encoded mutant p53 proteins. In two of those four cell lines where p53 expression was lower, point mutations were still detected. Thus, we conclude that overexpression of p53 is synonymous with mutation, but some mutations would not be detected by a simple immunohistochemical analysis. Mutation of the p53 gene is one of the commonest genetic changes in the development of human colorectal cancer. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 19 |
| Volume Number | 87 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1990-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Colonic Neoplasms Genetics Mutation Rectal Neoplasms Tumor Suppressor Protein P53 Adenocarcinoma Antibodies, Monoclonal Cell Line Enzyme-Linked Immunosorbent Assay Epitopes Immunoenzyme Techniques Molecular Sequence Data Oligonucleotide Probes Polymerase Chain Reaction RNA, Neoplasm Isolation & Purification Immunology Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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