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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Thomas, G. Ferrari, S. Totty, N. Kozma, S. C. Bassand, P. Siegmann, M. |
| Description | Author Affiliation: Kozma SC ( Friedrich Miescher Institute, Basel, Switzerland.); |
| Abstract | Recently we reported the purification of a mitogen-activated S6 kinase from Swiss mouse 3T3 fibroblasts and rat liver. The rat liver protein was cleaved with cyanogen bromide or trypsin and 17 of the resulting peptides were sequenced. DNA primers were generated from 3 peptides that had homology to sequences of the conserved catalytic domain of protein kinases. These primers were used in the polymerase chain reaction to obtain a 0.4-kilobase DNA fragment. This fragment was either radioactively labeled and hybridized to Northern blots of poly(A)+ mRNA or used to screen a rat liver cDNA library. Northern blot analysis revealed four transcripts of 2.5, 3.2, 4.0, and 6.0 kilobases, and five S6 kinase clones were obtained by screening the library. Only two of the clones, which were identical, encoded a full-length protein. This protein had a molecular weight of 56,160, which correlated closely to that of the dephosphorylated kinase determined by SDS/PAGE. The catalytic domain of the kinase resembles that of other serine/threonine kinases belonging to the second messenger subfamily of protein kinases. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 19 |
| Volume Number | 87 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1990-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Liver Enzymology Multigene Family Protein Kinases Genetics Second Messenger Systems Amino Acid Sequence Animals Cloning, Molecular Embryo, Mammalian Enzyme Activation Gene Library Mice Mitogens Pharmacology Molecular Sequence Data Poly A Isolation & Purification Polymerase Chain Reaction Metabolism RNA RNA, Messenger Restriction Mapping Ribosomal Protein S6 Kinases Sequence Homology, Nucleic Acid Comparative Study Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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