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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sancar, G. B. Sebastian, J. |
| Description | Author Affiliation: Sebastian J ( Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599-7260.); |
| Abstract | The PHR1 gene of Saccharomyces cerevisiae encodes the DNA repair enzyme photolyase. Transcription of PHR1 increases in response to treatment of cells with 254-nm radiation and chemical agents that damage DNA. We report here the identification of a damage-responsive DNA binding protein, termed photolyase regulatory protein (PRP), and its cognate binding site, termed the PHR1 upstream repression sequence, that together regulate induction of PHR1 transcription after DNA damage. PRP activity, monitored by electrophoretic-mobility-shift assay, was detected in cells during normal growth but disappeared within 30 min after irradiation. Copper-phenanthroline footprinting of PRP-DNA complexes revealed that PRP protects a 39-base-pair region of PHR1 5' flanking sequence beginning 40 base pairs upstream from the coding sequence. A prominent feature of the foot-printed region is a 22-base-pair palindrome. Deletion of the PHR1 upstream repression sequence increased the basal level expression of PHR1 in vivo and decreased induction after exposure of cells to UV radiation or methyl methanesulfonate, whereas insertion of the PRP binding site between the CYC1 upstream activation sequence and 'TATA' sequence reduced basal level expression and conferred damage responsiveness upon a reporter gene. Thus these observations establish that PRP is a damage-responsive repressor of PHR1 transcription. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 24 |
| Volume Number | 88 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1991-01-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | DNA Damage DNA Repair Genetics DNA-Binding Proteins Metabolism Deoxyribodipyrimidine Photo-Lyase Genes, Fungal Saccharomyces Cerevisiae Transcription, Genetic Binding Sites DNA, Fungal Drug Effects Radiation Effects Methyl Methanesulfonate Pharmacology Molecular Sequence Data Oligodeoxyribonucleotides Plasmids Promoter Regions, Genetic Restriction Mapping Enzymology Ultraviolet Rays Beta-Galactosidase Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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