NDLI: Cloning of the low-affinity murine granulocyte-macrophage colony-stimulating factor receptor and reconstitution of a high-affinity receptor complex.

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Light-induced phosphorylation of a membrane protein plays an early role in signal transduction for phototropism in Arabidopsis thaliana.

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Approximating Markov chains.

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Natural human antibodies to gamma interferon interfere with the immunomodulating activity of the lymphokine.

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Fatty acids activate a chimera of the clofibric acid-activated receptor and the glucocorticoid receptor.

Estrogen receptors colocalize with low-affinity nerve growth factor receptors in cholinergic neurons of the basal forebrain.

In vitro replication of adeno-associated virus DNA.

Finding errors in DNA sequences.

Leucine-responsive regulatory protein controls the expression of both the pap and fan pili operons in Escherichia coli.

Humanization of an anti-p185HER2 antibody for human cancer therapy.

Development of antibodies against the rat brain somatostatin receptor.

Homosynaptic long-term depression in area CA1 of hippocampus and effects of N-methyl-D-aspartate receptor blockade.

Retinal degeneration is rescued in transgenic rd mice by expression of the cGMP phosphodiesterase beta subunit.

Organization and evolution of the human epidermal keratinocyte transglutaminase I gene.

Cloning of the yeast FAS3 gene and primary structure of yeast acetyl-CoA carboxylase.

Amino acid substitutions in the sixth transmembrane domain of P-glycoprotein alter multidrug resistance.

Actions of dopamine and dopaminergic drugs on cloned serotonin receptors expressed in Xenopus oocytes.

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Cloning of the low-affinity murine granulocyte-macrophage colony-stimulating factor receptor and reconstitution of a high-affinity receptor complex.

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On the nature of global classification.

Theory of hydrophobicity: transient cavities in molecular liquids.

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Cloning of the low-affinity murine granulocyte-macrophage colony-stimulating factor receptor and reconstitution of a high-affinity receptor complex.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Martin, U. Luhr, S. Morrissey, P. J. Cosman, D. Larsen, A. Sorensen, R. Park, L. S.
Description	Author Affiliation: Park LS ( Immunex Research and Development Corporation, Seattle, WA 98101.);
Abstract	A cDNA clone (clone 71) that encodes a low-affinity receptor for murine granulocyte-macrophage colony-stimulating factor (GM-CSF) has been isolated by direct expression. This molecule is the homologue of the human GM-CSF receptor alpha subunit, although homology between these molecules is surprisingly low (less than 35% amino acid identity). The cDNA encodes a polypeptide of 387 amino acids, which contains the conserved features of the hematopoietin receptor superfamily. When expressed in COS-7 cells, this clone encodes a protein that binds radiolabeled murine GM-CSF with low affinity. Coexpression of clone 71 with a cDNA corresponding to a low-affinity interleukin 3 (IL-3) receptor (AIC2A) did not alter the affinity of binding of either GM-CSF or IL-3. However, coexpression of clone 71 with the IL-3 receptor-related cDNA AIC2B generated high-affinity binding sites for murine GM-CSF but not murine IL-3. These studies show that clone 71 and AIC2B are capable of forming an alpha beta complex capable of binding murine GM-CSF with high affinity, while AIC2A appears not to be a component of the murine GM-CSF receptor.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	10
Volume Number	89
Language	English
Publisher	National Academy of Sciences
Publisher Date	1992-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Receptors, Granulocyte-Macrophage Colony-Stimulating Factor Genetics Amino Acid Sequence Animals Cell Line Cloning, Molecular DNA Isolation & Purification Granulocyte-Macrophage Colony-Stimulating Factor Metabolism Interleukin-3 Kinetics Macromolecular Substances Mice Mice, Inbred C57BL Molecular Sequence Data Oligodeoxyribonucleotides RNA, Messenger Receptors, Interleukin-3 Recombinant Proteins Sequence Homology, Nucleic Acid Spleen Physiology Transfection Comparative Study Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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