NDLI: Mechanistic aspects of genome-wide demethylation in the preimplantation mouse embryo.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1993 Volume: 90 Issue: 24

Year: 1993 Volume: 90 Issue: 23

Year: 1993 Volume: 90 Issue: 22

Times to extinction for small populations of large birds.

Human immunodeficiency virus infection of human brain capillary endothelial cells occurs via a CD4/galactosylceramide-independent mechanism.

Neonatal imprinting predetermines the sexually dimorphic, estrogen-dependent expression of galanin in luteinizing hormone-releasing hormone neurons.

Inhibition of insulitis and prevention of diabetes in nonobese diabetic mice by blocking L-selectin and very late antigen 4 adhesion receptors.

Mitochondrial-genome-encoded RNAs: differential regulation by corticotropin in bovine adrenocortical cells.

Ion channels induced in lipid bilayers by subvirion particles of the nonenveloped mammalian reoviruses.

Insulin-like growth factor binding protein 1 stimulates cell migration and binds to the alpha 5 beta 1 integrin by means of its Arg-Gly-Asp sequence.

Giant multilevel cation channels formed by Alzheimer disease amyloid beta-protein [A beta P-(1-40)] in bilayer membranes.

Stable in vivo expression of the cystic fibrosis transmembrane conductance regulator with an adeno-associated virus vector.

A conserved helix motif complements the protein kinase core.

Identification of a hexapeptide that mimics a conformation-dependent binding site of acetylcholine receptor by use of a phage-epitope library.

Isolation of peptides that inhibit binding of basic fibroblast growth factor to its receptor from a random phage-epitope library.

Permeability properties of a large gated channel within the ferric enterobactin receptor, FepA.

The phylogenetically predicted base-pairing interaction between alpha and alpha' is required for group II splicing in vitro.

The gene for congenital chloride diarrhea maps close to but is distinct from the gene for cystic fibrosis transmembrane conductance regulator.

The human myelin basic protein gene is included within a 179-kilobase transcription unit: expression in the immune and central nervous systems.

Generation and screening of an oligonucleotide-encoded synthetic peptide library.

Expression of a Pim-1 transgene accelerates lymphoproliferation and inhibits apoptosis in lpr/lpr mice.

Biliary glycoprotein, a potential human cell adhesion molecule, is down-regulated in colorectal carcinomas.

Nitric oxide synthases reveal a role for calmodulin in controlling electron transfer.

Proteolytic processing and membrane association of putative nonstructural proteins of hepatitis C virus.

Myelin protein zero gene mutated in Charcot-Marie-tooth type 1B patients.

Uroguanylin: structure and activity of a second endogenous peptide that stimulates intestinal guanylate cyclase.

Decreased signaling competence as a result of receptor overexpression: overexpression of CD4 reduces its ability to activate p56lck tyrosine kinase and to regulate T-cell antigen receptor expression in immature CD4+CD8+ thymocytes.

Ablation of the prion protein (PrP) gene in mice prevents scrapie and facilitates production of anti-PrP antibodies.

Identification of a domain required for oncogenic activity and transcriptional suppression by v-erbA and thyroid-hormone receptor alpha.

Intragenic recombination at the human phosphoglucomutase 1 locus: predictions fulfilled.

The classical human phosphoglucomutase (PGM1) isozyme polymorphism is generated by intragenic recombination.

Thymocyte development in major histocompatibility complex-deficient mice: evidence for stochastic commitment to the CD4 and CD8 lineages.

Human bone marrow and umbilical cord blood cells generate CD4+ and CD8+ single-positive T cells in murine fetal thymus organ culture.

17 beta-estradiol mimics ligand activity of the c-erbB2 protooncogene product.

Failure of a protective major histocompatibility complex class II molecule to delete autoreactive T cells in autoimmune diabetes.

Individual subunits of bacterial luciferase are molten globules and interact with molecular chaperones.

Episodic evolution of RNA viruses.

Behavioral and magnetoencephalographic correlates of plasticity in the adult human brain.

Hepatocyte nuclear factor 3/fork head or 'winged helix' proteins: a family of transcription factors of diverse biologic function.

Punctuated equilibrium and positive Darwinian evolution in vesicular stomatitis virus.

Multiple oligomeric states regulate the DNA binding of helix-loop-helix peptides.

Interaction of glucocorticosteroid receptor and wild-type or mutated 90-kDa heat shock protein coexpressed in baculovirus-infected Sf9 cells.

Specific enhancement of beta-adrenergic receptor kinase activity by defined G-protein beta and gamma subunits.

Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface.

Functional expression and photoaffinity labeling of a cloned P2U purinergic receptor.

A method for producing monoclonal antibodies to human T-cell-receptor beta-chain variable regions.

Ligation of major histocompatibility complex class II molecules mediates apoptotic cell death in resting B lymphocytes.

Relationship between intrinsic connections and functional architecture revealed by optical imaging and in vivo targeted biocytin injections in primate striate cortex.

Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast.

Topography of the histone octamer surface: repeating structural motifs utilized in the docking of nucleosomal DNA.

Preferential repair of ionizing radiation-induced damage in the transcribed strand of an active human gene is defective in Cockayne syndrome.

Electroporation of mammalian skin: a mechanism to enhance transdermal drug delivery.

Identification of barriers to rotation of DNA segments in yeast from the topology of DNA rings excised by an inducible site-specific recombinase.

De novo synthesis of budding yeast DNA polymerase alpha and POL1 transcription at the G1/S boundary are not required for entrance into S phase.

Automatic enumeration and characterization of heterogeneous clonal progression in cell transformation.

Specific UV-induced mutation spectrum in the p53 gene of skin tumors from DNA-repair-deficient xeroderma pigmentosum patients.

RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frameshifting signal that directs the viral replicase synthesis.

Salmonella induces the formation of filamentous structures containing lysosomal membrane glycoproteins in epithelial cells.

Mechanistic aspects of genome-wide demethylation in the preimplantation mouse embryo.

Ectopic expression of wild-type or a dominant-negative mutant of transcription factor NTF-1 disrupts normal Drosophila development.

Comparative spectroscopic, calorimetric, and computational studies of nucleic acid complexes with 2',5'-versus 3',5'-phosphodiester linkages.

Expression of the human beta-amyloid precursor protein gene from a yeast artificial chromosome in transgenic mice.

A second hepatitis C virus-encoded proteinase.

The lysosomal proenzyme receptor that binds procathepsin L to microsomal membranes at pH 5 is a 43-kDa integral membrane protein.

Genetic analysis of homomeric interactions of human immunodeficiency virus type 1 integrase using the yeast two-hybrid system.

Multiple loci for synapse protein SNAP-25 in the tetraploid goldfish.

Isolation of a shark immunoglobulin light chain cDNA clone encoding a protein resembling mammalian kappa light chains: implications for the evolution of light chains.

Conservation of allelic richness in wild crop relatives is aided by assessment of genetic markers.

Chromatin structure and regulation of the eukaryotic regulatory gene GAL80.

Expression and biochemical properties of a protein serine/threonine phosphatase encoded by bacteriophage lambda.

Strand-invasion of duplex DNA by peptide nucleic acid oligomers.

Genetic and linguistic differentiation in the Americas.

Social control of primary sex differentiation in the Midas cichlid.

An examination of the generation-time effect on molecular evolution.

Comparison of somatic mutation in a transgenic versus host locus.

cDNA cloning, characterization, and tissue-specific expression of human xanthine dehydrogenase/xanthine oxidase.

Inhibition of vascular endothelial cell growth factor activity by an endogenously encoded soluble receptor.

RNA fingerprinting using arbitrarily primed PCR identifies differentially regulated RNAs in mink lung (Mv1Lu) cells growth arrested by transforming growth factor beta 1.

Cellular epigenetics: effects of passage history on competence of cells for 'spontaneous' transformation.

Resolution of the HLA-DRB6 puzzle: a case of grafting a de novo-generated exon on an existing gene.

Simple models for reading neuronal population codes.

Overexpression of human loricrin in transgenic mice produces a normal phenotype.

Direct transfer of transforming growth factor beta 1 gene into arteries stimulates fibrocellular hyperplasia.

Intraarticular expression of biologically active interleukin 1-receptor-antagonist protein by ex vivo gene transfer.

The precursor region of a protein active in sperm-egg fusion contains a metalloprotease and a disintegrin domain: structural, functional, and evolutionary implications.

Characterization of broken DNA molecules associated with V(D)J recombination.

Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite.

Efficacy of beta 1-adrenergic receptors is lower than that of beta 2-adrenergic receptors.

Acetalins: opioid receptor antagonists determined through the use of synthetic peptide combinatorial libraries.

Role of dimerization in yeast aspartyl-tRNA synthetase and importance of the class II invariant proline.

Yeast precursor mRNA processing protein PRP19 associates with the spliceosome concomitant with or just after dissociation of U4 small nuclear RNA.

Phosducin-like protein: an ethanol-responsive potential modulator of guanine nucleotide-binding protein function.

beta-Amyloid-(1-42) is a major component of cerebrovascular amyloid deposits: implications for the pathology of Alzheimer disease.

Brn-3.0: a POU-domain protein expressed in the sensory, immune, and endocrine systems that functions on elements distinct from known octamer motifs.

Heterotypic and homotypic associations between ezrin and moesin, two putative membrane-cytoskeletal linking proteins.

Highly identical cassettes of gene regulatory elements, genomically repetitive and present in RNA.

Cell growth and lambda phage development controlled by the same essential Escherichia coli gene, ftsH/hflB.

The Escherichia coli hflA locus encodes a putative GTP-binding protein and two membrane proteins, one of which contains a protease-like domain.

Gastric DNA-binding proteins recognize upstream sequence motifs of parietal cell-specific genes. (Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 90 Issue: 22)

Rapid assembly of the bacteriophage T4 core replication complex on a linear primer/template construct.

Evidence for linkage of the apolipoprotein A-II locus to plasma apolipoprotein A-II and free fatty acid levels in mice and humans.

A receptor tyrosine kinase found in breast carcinoma cells has an extracellular discoidin I-like domain. (Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 90 Issue: 22)

Year: 1993 Volume: 90 Issue: 21

Year: 1993 Volume: 90 Issue: 20

Year: 1993 Volume: 90 Issue: 19

Year: 1993 Volume: 90 Issue: 18

Year: 1993 Volume: 90 Issue: 17

Year: 1993 Volume: 90 Issue: 16

Year: 1993 Volume: 90 Issue: 15

Year: 1993 Volume: 90 Issue: 14

Year: 1993 Volume: 90 Issue: 13

Year: 1993 Volume: 90 Issue: 12

Year: 1993 Volume: 90 Issue: 11

Year: 1993 Volume: 90 Issue: 10

Year: 1993 Volume: 90 Issue: 9

Year: 1993 Volume: 90 Issue: 8

Year: 1993 Volume: 90 Issue: 7

Year: 1993 Volume: 90 Issue: 6

Year: 1993 Volume: 90 Issue: 5

Year: 1993 Volume: 90 Issue: 4

Year: 1993 Volume: 90 Issue: 3

Year: 1993 Volume: 90 Issue: 2

Year: 1993 Volume: 90 Issue: 1

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Mechanistic aspects of genome-wide demethylation in the preimplantation mouse embryo.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Razin, A. Gao, X. Kafri, T.
Description	Author Affiliation: Kafri T ( Department of Cellular Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel.);
Abstract	Gene-specific methylation patterns in mammals play a role in a variety of biological processes in the embryo and adult tissues. These patterns are established during embryo development by a process that involves genome-wide demethylation in the morula and de novo methylation in the pregastrula. To elucidate the mechanism of demethylation in the early mouse embryo, we have injected mouse zygotes with gene sequences that were methylated in vitro by Hpa II methylase and analyzed the methylation status of specific sites in blastocyst DNA. Because it had been propagated in Escherichia coli, the DNA used for these injections was also methylated at adenine residues in GATC sites. This allowed us to eliminate fully methylated, unintegrated DNA by Dpn I digestion and fully unmethylated, integrated DNA that underwent several rounds of replication by Mbo I digestion. The integrated, originally injected DNA strands were in a hemimethylated state and survived this treatment. The methylation status of Hpa II sites in these molecules was analyzed by Hpa II digestion of the genomic DNA isolated from blastocysts, followed by PCR amplification using appropriate primers. The results demonstrate that demethylation is achieved by an active mechanism and that specific sites in imprinted genes escape demethylation, maintaining a methylated state throughout preimplantation development.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	22
Volume Number	90
Language	English
Publisher	National Academy of Sciences
Publisher Date	1993-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	DNA Metabolism Mice Embryology Animals DNA Primers Embryonic Development Genes Hybridization, Genetic Imprinting (Psychology) Methylation Mice, Inbred BALB C Mice, Inbred C57BL Molecular Sequence Data Pregnancy Restriction Mapping Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in