NDLI: The LCB2 gene of Saccharomyces and the related LCB1 gene encode subunits of serine palmitoyltransferase, the initial enzyme in sphingolipid synthesis.

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The LCB2 gene of Saccharomyces and the related LCB1 gene encode subunits of serine palmitoyltransferase, the initial enzyme in sphingolipid synthesis.

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The LCB2 gene of Saccharomyces and the related LCB1 gene encode subunits of serine palmitoyltransferase, the initial enzyme in sphingolipid synthesis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lester, R. L. Wells, G. B. Nagiec, M. M. Baltisberger, J. A. Dickson, R. C.
Description	Author Affiliation: Nagiec MM ( Department of Biochemistry, University of Kentucky, Lexington 40536-0084.);
Abstract	The first and committed step in synthesis of the ceramide moiety of sphingolipids is catalyzed by serine palmitoyltransferase (EC 2.3.1.50), which condenses palmitoyl-CoA and serine to form 3-ketosphinganine. This step is thought to be tightly regulated to control the synthesis of sphingolipids, but data supporting this hypothesis are lacking mainly because the enzyme has resisted purification and consequent characterization. Rather than attempting to purify the enzyme from normal cells, we have taken a different tack and opted to try and overproduce the enzyme to facilitate its purification. Here we demonstrate that overproduction in Saccharomyces cerevisiae requires expression of LCB1, a previously isolated yeast gene, and LCB2, the isolation and characterization of which we describe. Several lines of evidence argue that both genes encode subunits of the enzyme; however, biochemical evidence will be needed to substantiate this hypothesis. Although overproduction was modest, 2- to 4-fold, it should now be possible to devise improved overproduction vectors for yeast or other host organisms.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	17
Volume Number	91
Language	English
Publisher	National Academy of Sciences
Publisher Date	1994-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Acyltransferases Biosynthesis Genetics Genes, Fungal Saccharomyces Cerevisiae Enzymology Sphingolipids Metabolism Amino Acid Sequence Gene Expression Genotype Macromolecular Substances Molecular Sequence Data Plasmids Restriction Mapping Saccharomyces Cerevisiae Proteins Sequence Homology, Amino Acid Serine C-Palmitoyltransferase Comparative Study Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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