NDLI: Identification of kappa opioid receptors in the immune system by indirect immunofluorescence.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1995 Volume: 92 Issue: 26

Year: 1995 Volume: 92 Issue: 25

Year: 1995 Volume: 92 Issue: 24

Year: 1995 Volume: 92 Issue: 23

Year: 1995 Volume: 92 Issue: 22

Year: 1995 Volume: 92 Issue: 21

Year: 1995 Volume: 92 Issue: 20

Year: 1995 Volume: 92 Issue: 19

Year: 1995 Volume: 92 Issue: 18

Year: 1995 Volume: 92 Issue: 17

Year: 1995 Volume: 92 Issue: 16

Year: 1995 Volume: 92 Issue: 15

Year: 1995 Volume: 92 Issue: 14

Year: 1995 Volume: 92 Issue: 13

Year: 1995 Volume: 92 Issue: 12

Year: 1995 Volume: 92 Issue: 11

Year: 1995 Volume: 92 Issue: 10

Year: 1995 Volume: 92 Issue: 9

Year: 1995 Volume: 92 Issue: 8

Year: 1995 Volume: 92 Issue: 7

Year: 1995 Volume: 92 Issue: 6

Year: 1995 Volume: 92 Issue: 5

Year: 1995 Volume: 92 Issue: 4

A linear Ricardo model with varying parameters.

Vasopressin increases water permeability of kidney collecting duct by inducing translocation of aquaporin-CD water channels to plasma membrane.

Gene therapy inhibiting neointimal vascular lesion: in vivo transfer of endothelial cell nitric oxide synthase gene.

The structure of unliganded reverse transcriptase from the human immunodeficiency virus type 1.

Two closely linked but separable promoters for human neuronal nitric oxide synthase gene transcription.

Thymidine phosphorylase is angiogenic and promotes tumor growth.

A sauvagine/corticotropin-releasing factor receptor expressed in heart and skeletal muscle.

Hepatitis B virus transactivator protein X interacts with the TATA-binding protein.

Single cell monitoring of growth arrest and morphological changes induced by transfer of wild-type p53 alleles to glioblastoma cells.

Rearrangements in the genome of the bacterium Salmonella typhi.

Gene therapy for hemophilia A: production of therapeutic levels of human factor VIII in vivo in mice.

Characterization of the genomic breakpoint and chimeric transcripts in the EWS-WT1 gene fusion of desmoplastic small round cell tumor.

Application of chiral gas chromatography with electroantennographic detection to the determination of the stereochemistry of a cockroach sex pheromone.

Structure, stereochemistry, and thermal isomerization of the male sex pheromone of the longhorn beetle Anaglyptus subfasciatus.

Subunit functional studies of NAD(P)H:quinone oxidoreductase with a heterodimer approach.

Purification and characterization of recombinant human p50csk protein-tyrosine kinase from an Escherichia coli expression system overproducing the bacterial chaperones GroES and GroEL.

Contributions made by individual methylation sites of the Escherichia coli aspartate receptor to chemotactic behavior.

Development of leukemia in mice transgenic for the tax gene of human T-cell leukemia virus type I.

Identification of kappa opioid receptors in the immune system by indirect immunofluorescence.

Oxidation of gamma II-crystallin solutions yields dimers with a high phase separation temperature.

The canine betaine gamma-amino-n-butyric acid transporter gene: diverse mRNA isoforms are regulated by hypertonicity and are expressed in a tissue-specific manner.

Platelet-derived growth factor triggers translocation of the insulin-regulatable glucose transporter (type 4) predominantly through phosphatidylinositol 3-kinase binding sites on the receptor.

Internal and interfacial dielectric properties of cytochrome c from molecular dynamics in aqueous solution.

Cooperative action of cellular proteins YB-1 and Pur alpha with the tumor antigen of the human JC polyomavirus determines their interaction with the viral lytic control element.

Cell cycle-dependent regulation of p185neu: a relationship between disruption of this regulation and transformation.

Determination of absolute amounts of GDP and GTP bound to Ras in mammalian cells: comparison of parental and Ras-overproducing NIH 3T3 fibroblasts.

Identification of presumed ancestral DNA sequences of phaseolin in Phaseolus vulgaris.

Sexual dimorphism in the architecture of the lung's gas-exchange region.

Directed hydroxyl radical probing of 16S rRNA using Fe(II) tethered to ribosomal protein S4.

Stringent control and growth-rate-dependent control have nonidentical promoter sequence requirements.

Structural hierarchy in the clustering of HLA class I molecules in the plasma membrane of human lymphoblastoid cells.

Quinoxalines block the mechanism of directional selectivity in ganglion cells of the rabbit retina.

Immunotoxin-mediated conditional disruption of specific neurons in transgenic mice.

Expression cloning of cardiotrophin 1, a cytokine that induces cardiac myocyte hypertrophy.

Development and characterization of essential fatty acid deficiency in human endothelial cells in culture.

Hypermutability of mouse chromosome 2 during the development of x-ray-induced murine myeloid leukemia.

Modulation of protein tyrosine phosphorylation in rat insular cortex after conditioned taste aversion training.

Retrovirus-mediated transfer of an angiotensin type I receptor (AT1-R) antisense sequence decreases AT1-Rs and angiotensin II action in astroglial and neuronal cells in primary cultures from the brain.

Targeting the maize T-urf13 product into tobacco mitochondria confers methomyl sensitivity to mitochondrial respiration.

A common fold for peptide synthetases cleaving ATP to ADP: glutathione synthetase and D-alanine:d-alanine ligase of Escherichia coli.

Induction of heme oxygenase 1 in the retina by intense visible light: suppression by the antioxidant dimethylthiourea.

G1 cyclin-dependent activation of p34CDC28 (Cdc28p) in vitro.

Disruption of the nucleoporin gene NUP133 results in clustering of nuclear pore complexes.

Paternal investment affects prevalence of malaria.

Passive immunotherapy in AIDS: a double-blind randomized study based on transfusions of plasma rich in anti-human immunodeficiency virus 1 antibodies vs. transfusions of seronegative plasma.

Positive selection of invariant V alpha 14+ T cells by non-major histocompatibility complex-encoded class I-like molecules expressed on bone marrow-derived cells.

Hydrogen bonding in water using synthetic receptors.

Identification of galectin-3 as a factor in pre-mRNA splicing.

The three-dimensional structure of a class I major histocompatibility complex molecule missing the alpha 3 domain of the heavy chain.

Contribution of cotranslational folding to the rate of formation of native protein structure.

Amino acid sequence of rat kidney glutathione synthetase.

Conservation of deposition-related acetylation sites in newly synthesized histones H3 and H4.

Independent regulation of sterol regulatory element-binding proteins 1 and 2 in hamster liver.

cDNA cloning of prophenoloxidase from the freshwater crayfish Pacifastacus leniusculus and its activation.

MARCKS deficiency in mice leads to abnormal brain development and perinatal death.

A single point mutation leading to loss of catalytic activity in human thiopurine S-methyltransferase.

Superoxide dismutase is an abundant component in cell bodies, dendrites, and axons of motor neurons and in a subset of other neurons.

A single arginine residue determines species specificity of the human growth hormone receptor.

Maize branching enzyme catalyzes synthesis of glycogen-like polysaccharide in glgB-deficient Escherichia coli.

The minor form alpha' chain from lamprey fibrinogen is rapidly crosslinked during clotting.

Transfection of Plasmodium falciparum within human red blood cells.

Adherence of human immunodeficiency virus-infected lymphocytes to fetal placental cells: a model of maternal --> fetal transmission.

Decreased muscle glucose transport/phosphorylation is an early defect in the pathogenesis of non-insulin-dependent diabetes mellitus.

Visual latencies in cytochrome oxidase bands of macaque area V2.

A motion area in human visual cortex.

Year: 1995 Volume: 92 Issue: 3

Year: 1995 Volume: 92 Issue: 2

Year: 1995 Volume: 92 Issue: 1

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Identification of kappa opioid receptors in the immune system by indirect immunofluorescence.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lawrence, D. M. El-hamouly, W. Leary, J. F. Archer, S. Bidlack, J. M.
Description	Author Affiliation: Lawrence DM ( Department of Pharmacology, University of Rochester, School of Medicine and Dentistry, NY 14642.);
Abstract	A method to visualize the kappa opioid receptor is described that uses a high-affinity fluorescein-conjugated opioid ligand and indirect immunofluorescence with the phycoerythrin fluorophore to amplify the signal. The mouse thymoma cell line R1E/TL8x.1.G1.OUAr.1 (R1EGO), which expresses the kappa 1 but not mu or delta opioid receptors, was used as a positive control for fluorescence labeling. A fluorescein isothiocyanate-conjugated arylacetamide (FITC-AA) compound displaying high affinity for the kappa opioid receptor was synthesized. R1EGO cells were incubated with FITC-AA, in the absence or presence of the kappa-selective opioid antagonist nor-binaltorphimine (nor-BNI) as a competitor. By using fluorescence microscopy and flow cytometry, incubation of R1EGO cells with FITC-AA alone was not sufficient for the detection of specific staining of the kappa opioid receptor. To amplify the FITC-AA fluorescence, the fluorescein served as a hapten for subsequent antibody detection. R1EGO cells were incubated with FITC-AA, followed by biotinylated rabbit anti-fluorescein IgG and extravidin-conjugated R-phycoerythrin. By using this approach, R1EGO cells were stained with phycoerythrin-amplified FITC-AA, and the staining was displaced with nor-BNI. Flow cytometry showed that titrations of both FITC-AA and nor-BNI produced saturable concentration-dependent changes in the median phycoerythrin fluorescence intensity, with optimal staining at 30 microM FITC-AA. Up to 80% of the fluorescence above background was inhibited by nor-BNI. Freshly isolated thymocytes from C57BL/6ByJ mice also showed nor-BNI-sensitive staining with the FITC-AA amplification. This sensitive method of indirect phycoerythrin immunofluorescence can be used to amplify any fluorescein-conjugated opioid ligand for the detection of opioid receptors.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	4
Volume Number	92
Language	English
Publisher	National Academy of Sciences
Publisher Date	1995-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Immune System Chemistry Receptors, Opioid, Kappa Acetamides Amino Acid Sequence Animals Fluorescein-5-isothiocyanate Fluorescent Antibody Technique Mice Mice, Inbred C57BL Molecular Sequence Data Naltrexone Analogs & Derivatives Tumor Cells, Cultured Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in