NDLI: Localization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells.

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Quasars and active galactic nuclei: high resolution radio imaging.

Very-long-baseline radio interferometry surveys of the compact structure in active galactic nuclei.

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Structure and evolution of the compact radio source in NGC 1275.

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Subparsec-scale structure and evolution of Centaurus A (NGC5128).

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Localization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells.

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Localization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yasuda, K. Tsuji, F. I. Ogawa, H. Inouye, S. Umesono, K.
Description	Author Affiliation: Ogawa H ( Graduate School of Biological Sciences, Nara Institute of Science and Technology, Japan.);
Abstract	The localization, trafficking, and fluorescence of Aequorea green fluorescent protein (GFP) in cultured vertebrate cells transiently transfected with GFP cDNA were studied. Fluorescence of GFP in UV light was found to be strongest when cells were incubated at 30 degrees C but was barely visible at an incubation temperature of 37 degrees C. COS-1 cells, primary chicken embryonic retina cells, and carp epithelial cells were fluorescently labeled under these conditions. GFP was distributed uniformly throughout the cytoplasm and nucleus independent of cell type examined. When GFP was fused to PML protooncogene product, fluorescence was detected in a unique nuclear organelle pattern indistinguishable from that of PML protein, showing the potential use of GFP as a fluorescent tag. To analyze both function and intracellular trafficking of proteins fused to GFP, a GFP-human glucocorticoid receptor fusion construct was prepared. The GFP-human glucocorticoid receptor efficiently transactivated the mouse mammary tumor virus promoter in response to dexamethasone at 30 degrees C but not at 37 degrees C, indicating that temperature is important, even for function of the GFP fusion protein. The dexamethasone-induced translocation of GFP-human glucocorticoid receptor from cytoplasm to nucleus was complete within 15 min; the translocation could be monitored in a single living cell in real time.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	25
Volume Number	92
Language	English
Publisher	National Academy of Sciences
Publisher Date	1995-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Biological Markers Cell Compartmentation Genes, Reporter Luminescent Proteins Metabolism Neoplasm Proteins Nuclear Proteins Amino Acid Sequence Animals Biological Transport Cells, Cultured Dexamethasone Pharmacology Green Fluorescent Proteins Genetics Microscopy, Fluorescence Molecular Sequence Data Receptors, Glucocorticoid Recombinant Fusion Proteins Temperature Transcription Factors Transcription, Genetic Transcriptional Activation Tumor Suppressor Proteins Vertebrates Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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