NDLI: A highly active decarboxylating dehydrogenase with rationally inverted coenzyme specificity.

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Quasars and active galactic nuclei: high resolution radio imaging.

Very-long-baseline radio interferometry surveys of the compact structure in active galactic nuclei.

Morphology of high-luminosity compact radio sources.

Very-long-baseline radio interferometry observations of low power radio galaxies.

Structure and evolution of the compact radio source in NGC 1275.

The parsec-scale jet in M87.

Subparsec-scale structure and evolution of Centaurus A (NGC5128).

The nuclear jet and counterjet region of the radio galaxy Cygnus A.

The nuclear region of the spiral galaxy M81.

Very-long-baseline radio interferometry (VLBI) observations of gamma-ray blazars: results from millimeter-VLBI observations.

Bidirectional motion observed in the compact symmetric object 1946+708.

Superluminal sources.

Grs 1915+105: a superluminal source in the Galaxy.

Possible links between BL Lacertae objects and quasars from very long baseline interferometry radio data.

The case for unification.

Difficulties with unification.

Unification and large-scale structure.

Compact steep-spectrum sources and the unified scheme.

Optical evidence for the unification of active galactic nuclei and quasi-stellar objects.

Probing active galactic nuclei with H2O megamasers.

Gravitational lensing of active galactic nuclei.

Probes of the inner jets of blazars.

The acceleration and collimation of jets.

Evolution of powerful extragalactic radio sources.

Lipoxygenase-catalyzed oxygenation of storage lipids is implicated in lipid mobilization during germination.

Plant histochemistry by correlation peak imaging.

Radial versus tangential migration of neuronal clones in the developing cerebral cortex.

The histone fold: evolutionary questions.

Uniparental inheritance of mitochondrial and chloroplast genes: mechanisms and evolution.

Sequence verification of human creatine kinase (43 kDa) isozymes by high-resolution tandem mass spectrometry. (Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 92 Issue: 25)

Identification of a hexapeptide inhibitor of the human immunodeficiency virus integrase protein by using a combinatorial chemical library.

In vitro trans-splicing in Saccharomyces cerevisiae.

A protein that interacts with members of the nuclear hormone receptor family: identification and cDNA cloning.

Enhanced somatic mutation rates induced in stem cells of mice by low chronic exposure to ethylnitrosourea.

Identification of a Drosophila G protein alpha subunit (dGq alpha-3) expressed in chemosensory cells and central neurons.

Discovery of adrenomedullin in rat ischemic cortex and evidence for its role in exacerbating focal brain ischemic damage.

Equine severe combined immunodeficiency: a defect in V(D)J recombination and DNA-dependent protein kinase activity.

Studies of group B streptococcal infection in mice deficient in complement component C3 or C4 demonstrate an essential role for complement in both innate and acquired immunity.

A de novo missense mutation of the beta subunit of the epithelial sodium channel causes hypertension and Liddle syndrome, identifying a proline-rich segment critical for regulation of channel activity.

Transport of cytoskeletal elements in the squid giant axon.

Red/far-red and blue light-responsive regions of maize rbcS-m3 are active in bundle sheath and mesophyll cells, respectively.

Isolation of virus-neutralizing RNAs from a large pool of random sequences.

Inducible nitric oxide synthase: identification of amino acid residues essential for dimerization and binding of tetrahydrobiopterin.

Proton transport by a bacteriorhodopsin mutant, aspartic acid-85-->asparagine, initiated in the unprotonated Schiff base state.

An amino acid sequence motif sufficient for subnuclear localization of an arginine/serine-rich splicing factor.

Induction of a Cryphonectria parasitica cellobiohydrolase I gene is suppressed by hypovirus infection and regulated by a GTP-binding-protein-linked signaling pathway involved in fungal pathogenesis.

Regulation of microfilament organization and anchorage-independent growth by tropomyosin 1.

14-3-3 proteins: potential roles in vesicular transport and Ras signaling in Saccharomyces cerevisiae.

A potent transrepression domain in the retinoblastoma protein induces a cell cycle arrest when bound to E2F sites.

Microsatellite variability and genetic distances.

Systemic immunization with papillomavirus L1 protein completely prevents the development of viral mucosal papillomas.

The CD19 signal transduction molecule is a response regulator of B-lymphocyte differentiation.

Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5' proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development.

Temporal fluctuations in coherence of brain waves.

An apical permeability barrier to NH3/NH4+ in isolated, perfused colonic crypts.

Projection structure of frog rhodopsin in two crystal forms.

Coupling the phosphotransferase system and the methyl-accepting chemotaxis protein-dependent chemotaxis signaling pathways of Escherichia coli.

Escherichia coli transcript cleavage factors GreA and GreB stimulate promoter escape and gene expression in vivo and in vitro.

Thyroid hormone (T3) inhibits ciprofibrate-induced transcription of genes encoding beta-oxidation enzymes: cross talk between peroxisome proliferator and T3 signaling pathways.

Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor.

Phosphinate analogs of D-, D-dipeptides: slow-binding inhibition and proteolysis protection of VanX, a D-, D-dipeptidase required for vancomycin resistance in Enterococcus faecium.

A mouse model for beta 0-thalassemia.

Phosphatidylinositol 3-kinase binding to polyoma virus middle tumor antigen mediates elevation of glucose transport by increasing translocation of the GLUT1 transporter.

Cloning and characterization of Lnk, a signal transduction protein that links T-cell receptor activation signal to phospholipase C gamma 1, Grb2, and phosphatidylinositol 3-kinase.

Inhibition of NF-AT-dependent transcription by NF-kappa B: implications for differential gene expression in T helper cell subsets.

FAS is highly expressed in the germinal center but is not required for regulation of the B-cell response to antigen.

Characterization of a 23-kDa protein associated with CD40.

Cooperative interactions among afferents govern the induction of homosynaptic long-term depression in the hippocampus.

Identification of a receptor/G-protein contact site critical for signaling specificity and G-protein activation.

Molecular cloning of a preprohormone from sea anemones containing numerous copies of a metamorphosis-inducing neuropeptide: a likely role for dipeptidyl aminopeptidase in neuropeptide precursor processing.

Purification, cloning, and functional expression of sucrose:fructan 6-fructosyltransferase, a key enzyme of fructan synthesis in barley.

Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes.

Phylogenetic relationships of the hoatzin, an enigmatic South American bird.

A highly active decarboxylating dehydrogenase with rationally inverted coenzyme specificity.

Engineering an intracellular pathway for major histocompatibility complex class II presentation of antigens.

Molecular heterogeneity of progenitors and radial migration in the developing cerebral cortex revealed by transgene expression.

The 'DEAD box' protein DbpA interacts specifically with the peptidyltransferase center in 23S rRNA.

The LAR/PTP delta/PTP sigma subfamily of transmembrane protein-tyrosine-phosphatases: multiple human LAR, PTP delta, and PTP sigma isoforms are expressed in a tissue-specific manner and associate with the LAR-interacting protein LIP.1.

Specific mutations in the ligand binding domain selectively abolish the silencing function of human thyroid hormone receptor beta.

Structural and functional analysis of pp70S6k.

Mapping by multifragment cloning in vivo.

Isolation of yeast artificial chromosomes free of endogenous yeast chromosomes: construction of alternate hosts with defined karyotypic alterations.

Primary structure and functional expression of a cGMP-gated potassium channel.

Protein kinase A-independent modulation of ion channels in the brain by cyclic AMP.

Crystal structure of the complex of a catalytic antibody Fab fragment with a transition state analog: structural similarities in esterase-like catalytic antibodies.

Assembly of regularly spaced nucleosome arrays by Drosophila chromatin assembly factor 1 and a 56-kDa histone-binding protein.

Specific activation in jun-transformed avian fibroblasts of a gene (bkj) related to the avian beta-keratin gene family.

cAMP-dependent SOS induction and mutagenesis in resting bacterial populations.

Inferring identify from DNA profile evidence.

The Bcr-Abl leukemia oncogene activates Jun kinase and requires Jun for transformation.

A single amino acid in gamma-aminobutyric acid rho 1 receptors affects competitive and noncompetitive components of picrotoxin inhibition.

Cloning of a gamma-aminobutyric acid type C receptor subunit in rat retina with a methionine residue critical for picrotoxinin channel block.

The specificity of the transforming growth factor beta receptor kinases determined by a spatially addressable peptide library.

Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase IIx isozyme.

Expression of human inducible nitric oxide synthase in a tetrahydrobiopterin (H4B)-deficient cell line: H4B promotes assembly of enzyme subunits into an active dimer.

Mutagenesis of palmitoylation sites in endothelial nitric oxide synthase identifies a novel motif for dual acylation and subcellular targeting.

A role for Rho in Ras transformation.

Arabidopsis mutants deficient in T-DNA integration.

Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint.

Susceptibility of cloned K+ channels to reactive oxygen species.

Topoisomerase IV is a target of quinolones in Escherichia coli.

Recovery of respiration following the SOS response of Escherichia coli requires RecA-mediated induction of 2-keto-4-hydroxyglutarate aldolase.

Human neoplasms elicit multiple specific immune responses in the autologous host.

CD38 ligation induces tyrosine phosphorylation of Bruton tyrosine kinase and enhanced expression of interleukin 5-receptor alpha chain: synergistic effects with interleukin 5.

Localization of choline acetyltransferase in rat peripheral sympathetic neurons and its coexistence with nitric oxide synthase and neuropeptides.

MyoD forms micelles which can dissociate to form heterodimers with E47: implications of micellization on function.

Characterization of repetitive DNA in the Mycoplasma genitalium genome: possible role in the generation of antigenic variation.

An anatomical substrate for experience-dependent plasticity of the rat barrel field cortex.

Molecular determinants of drug access to the receptor site for antiarrhythmic drugs in the cardiac Na+ channel.

Differential sensitivity of stilbenedisulfonates in their reaction with band 3 HT (Pro-868-->Leu).

Evidence for DNA phosphate backbone alkylation and cleavage by pyrrolo[1,2-a]benzimidazoles: small molecules capable of causing base-pair-specific phosphodiester bond hydrolysis.

Analysis of RNA-binding proteins by in vitro genetic selection: identification of an amino acid residue important for locking U1A onto its RNA target.

Core promoter-specific function of a mutant transcription factor TFIID defective in TATA-box binding.

Ascorbic acid is essential for the release of insulin from scorbutic guinea pig pancreatic islets.

Inflammatory skin disease in transgenic mice that express high levels of interleukin 1 alpha in basal epidermis.

Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain.

Haploidy and androgenesis in Drosophila.

Human immunodeficiency virus 1 envelope-initiated G2-phase programmed cell death.

A Fas-associated protein factor, FAF1, potentiates Fas-mediated apoptosis.

Localization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells.

Investigation of the prebiotic synthesis of amino acids and RNA bases from CO2 using FeS/H2S as a reducing agent.

Expression studies of catalytic antibodies.

GAIP, a protein that specifically interacts with the trimeric G protein G alpha i3, is a member of a protein family with a highly conserved core domain.

Sequence similarity analysis of Escherichia coli proteins: functional and evolutionary implications.

Cytochrome P450 1A1 promoter as a genetic switch for the regulatable and physiological expression of a plasma protein in transgenic mice.

Defective IgE production by SJL mice is linked to the absence of CD4+, NK1.1+ T cells that promptly produce interleukin 4.

Structural basis for major histocompatibility complex (MHC)-linked susceptibility to autoimmunity: charged residues of a single MHC binding pocket confer selective presentation of self-peptides in pemphigus vulgaris.

Production of avian leukosis virus particles in mammalian cells can be mediated by the interaction of the human immunodeficiency virus protein Rev and the Rev-responsive element.

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A highly active decarboxylating dehydrogenase with rationally inverted coenzyme specificity.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, R. Dean, A. M. Greer, A.
Description	Author Affiliation: Chen R ( Department of Biological Chemistry, Chicago Medical School, North Chicago, IL 60064, USA.);
Abstract	The isocitrate dehydrogenase of Escherichia coli, which lacks the Rossmann fold common to other dehydrogenases, displays a 7000-fold preference for NADP over NAD (calculated as the ratio of kcat/Km). Guided by x-ray crystal structures and molecular modeling, site-directed mutagenesis has been used to introduce six substitutions in the adenosine binding pocket that systematically shift coenzyme preference toward NAD. The engineered enzyme displays an 850-fold preference for NAD over NADP, which exceeds the 140-fold preference displayed by a homologous NAD-dependent enzyme. Of the six mutations introduced, only one is identical in all related NAD-dependent enzyme sequences--strict adherence to homology as a criterion for replacing these amino acids impairs function. Two additional mutations at remote sites improve performance further, resulting in a final mutant enzyme with kinetic characteristics and coenzyme preference comparable to naturally occurring homologous NAD-dependent enzymes.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	25
Volume Number	92
Language	English
Publisher	National Academy of Sciences
Publisher Date	1995-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Escherichia Coli Genetics Isocitrate Dehydrogenase NADP Metabolism NAD Protein Engineering Amino Acid Sequence Binding Sites Enzymology Kinetics Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Substrate Specificity Comparative Study Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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