NDLI: Structure and function in rhodopsin: correct folding and misfolding in point mutants at and in proximity to the site of the retinitis pigmentosa mutation Leu-125-->Arg in the transmembrane helix C.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1996 Volume: 93 Issue: 26

Year: 1996 Volume: 93 Issue: 25

Year: 1996 Volume: 93 Issue: 24

Year: 1996 Volume: 93 Issue: 23

Year: 1996 Volume: 93 Issue: 22

Year: 1996 Volume: 93 Issue: 21

Year: 1996 Volume: 93 Issue: 20

Year: 1996 Volume: 93 Issue: 19

Year: 1996 Volume: 93 Issue: 18

Year: 1996 Volume: 93 Issue: 17

Year: 1996 Volume: 93 Issue: 16

Year: 1996 Volume: 93 Issue: 15

Year: 1996 Volume: 93 Issue: 14

Year: 1996 Volume: 93 Issue: 13

Year: 1996 Volume: 93 Issue: 12

Year: 1996 Volume: 93 Issue: 11

Year: 1996 Volume: 93 Issue: 10

Mapping leaf surface landscapes.

Nest predation by cowbirds and its consequences for passerine demography.

Culture as shared cognitive representations.

Ediacaran biota from Sonora, Mexico.

Why fisheries collapse and what to do about it.

Distribution of spontaneous plant hybrids.

Origin of genetic code: A needle in the haystack of tRNA sequences.

Does DG42 synthesize hyaluronan or chitin?: A controversy about oligosaccharides in vertebrate development.

Retinitis pigmentosa: unfolding its mystery.

Onconeural antigens and the paraneoplastic neurologic disorders: at the intersection of cancer, immunity, and the brain.

The presence of codon-anticodon pairs in the acceptor stem of tRNAs.

Cells expressing the DG42 gene from early Xenopus embryos synthesize hyaluronan.

Homologs of the Xenopus developmental gene DG42 are present in zebrafish and mouse and are involved in the synthesis of Nod-like chitin oligosaccharides during early embryogenesis.

Structure and function in rhodopsin: correct folding and misfolding in two point mutants in the intradiscal domain of rhodopsin identified in retinitis pigmentosa.

Structure and function in rhodopsin: correct folding and misfolding in point mutants at and in proximity to the site of the retinitis pigmentosa mutation Leu-125-->Arg in the transmembrane helix C.

Role of neuronal nitric oxide in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopaminergic neurotoxicity.

Functional interaction and colocalization of the herpes simplex virus 1 major regulatory protein ICP4 with EAP, a nucleolar-ribosomal protein.

Single cell Ca2+/cAMP cross-talk monitored by simultaneous Ca2+/cAMP fluorescence ratio imaging.

Antigenic peptides containing large PEG loops designed to extend out of the HLA-A2 binding site form stable complexes with class I major histocompatibility complex molecules.

Viral cross talk: intracellular inactivation of the hepatitis B virus during an unrelated viral infection of the liver.

Trafficking of Plasmodium chabaudi adami-infected erythrocytes within the mouse spleen.

Controlling protein association and subcellular localization with a synthetic ligand that induces heterodimerization of proteins.

The hard-soft acid-base principle in enzymatic catalysis: dual reactivity of phosphoenolpyruvate.

The platelet-derived growth factor alpha-receptor is encoded by a growth-arrest-specific (gas) gene.

Myocyte-specific enhancer factor 2 acts cooperatively with a muscle activator region to regulate Drosophila tropomyosin gene muscle expression.

Genomic structure of the human retinoblastoma-related Rb2/p130 gene.

Regulation of the retinoblastoma protein-related protein p107 by G1 cyclin-associated kinases.

Expression cloning of a cDNA for human ceramide glucosyltransferase that catalyzes the first glycosylation step of glycosphingolipid synthesis. (Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 93 Issue: 10)

Peroxo-iron and oxenoid-iron species as alternative oxygenating agents in cytochrome P450-catalyzed reactions: switching by threonine-302 to alanine mutagenesis of cytochrome P450 2B4.

Signal transduction in the archaeon Halobacterium salinarium is processed through three subfamilies of 13 soluble and membrane-bound transducer proteins.

A circadian clock regulates rod and cone input to fish retinal cone horizontal cells.

Cloning and functional expression of cDNAs encoding human and rat pancreatic polypeptide receptors.

Dexamethasone responsiveness of a major glucocorticoid-inducible CYP3A gene is mediated by elements unrelated to a glucocorticoid receptor binding motif.

A general additive distance with time-reversibility and rate variation among nucleotide sites.

Responses of the phototransduction cascade to dim light.

Activated Ras signals differentiation and expansion of CD4+8+ thymocytes.

Excitotoxicity in the lung: N-methyl-D-aspartate-induced, nitric oxide-dependent, pulmonary edema is attenuated by vasoactive intestinal peptide and by inhibitors of poly(ADP-ribose) polymerase.

Hippocampal acetylcholine release during memory testing in rats: augmentation by glucose.

Reconstitution of the hippocampal mossy fiber and associational-commissural pathways in a novel dissociated cell culture system.

A presynaptic locus for long-term potentiation of elementary synaptic transmission at mossy fiber synapses in culture.

Arabidopsis mutant analysis and gene regulation define a nonredundant role for glutamate dehydrogenase in nitrogen assimilation.

DNA sequencing by delayed extraction-matrix-assisted laser desorption/ionization time of flight mass spectrometry.

Mos/mitogen-activated protein kinase can induce early meiotic phenotypes in the absence of maturation-promoting factor: a novel system for analyzing spindle formation during meiosis I.

Polynitrosylated proteins: characterization, bioactivity, and functional consequences.

The control of hematopoiesis and leukemia: from basic biology to the clinic.

Behavioral stress modifies hippocampal plasticity through N-methyl-D-aspartate receptor activation.

Sequence-specific DNA-binding dominated by dehydration.

Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release.

Age-related losses of cognitive function and motor skills in mice are associated with oxidative protein damage in the brain.

Selective attention to stimulus location modulates the steady-state visual evoked potential.

Genetic interactions among cytoplasmic dynein, dynactin, and nuclear distribution mutants of Neurospora crassa.

p53 as a target for cancer vaccines: recombinant canarypox virus vectors expressing p53 protect mice against lethal tumor cell challenge.

Targeted replacement of the mycocerosic acid synthase gene in Mycobacterium bovis BCG produces a mutant that lacks mycosides.

Dimerization specificity of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA, and AGAMOUS.

High resolution ultrastructural mapping of total calcium: electron spectroscopic imaging/electron energy loss spectroscopy analysis of a physically/chemically processed nerve-muscle preparation.

The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.

Bc1-2 protects mice against fatal alphavirus encephalitis.

Prostaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect.

Predominant role of alpha 4-integrins for distinct steps of lymphoma metastasis.

Sensitivity and selectivity of the DNA damage sensor responsible for activating p53-dependent G1 arrest.

Neuregulins with an Ig-like domain are essential for mouse myocardial and neuronal development.

Huntingtin-associated protein (HAP1): discrete neuronal localizations in the brain resemble those of neuronal nitric oxide synthase.

Visualization of glucocorticoid receptor translocation and intranuclear organization in living cells with a green fluorescent protein chimera.

Oligodeoxynucleotides inhibit retinal neovascularization in a murine model of proliferative retinopathy.

How cellular slime molds evade nematodes.

Crystal structure of a human TATA box-binding protein/TATA element complex.

Two Arabidopsis cyclin promoters mediate distinctive transcriptional oscillation in synchronized tobacco BY-2 cells.

Novel retinoic acid receptor ligands in Xenopus embryos.

4-Oxoretinol, a new natural ligand and transactivator of the retinoic acid receptors.

Elevated interleukin 6 is induced by prostaglandin E2 in a murine model of inflammation: possible role of cyclooxygenase-2.

Complementation analysis of mutants of nitric oxide synthase reveals that the active site requires two hemes.

Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers.

In vivo suppression of the renal Na+/Pi cotransporter by antisense oligonucleotides.

Using ubiquitin to follow the metabolic fate of a protein.

DNA analysis and diagnostics on oligonucleotide microchips.

Internal cleavage of the inhibitory 7B2 carboxyl-terminal peptide by PC2: a potential mechanism for its inactivation.

Translational regulation of mammalian and Drosophila citric acid cycle enzymes via iron-responsive elements.

Expression of transduced genes in mice generated by infecting blastocysts with avian leukosis virus-based retroviral vectors.

Two-dimensional protein crystallization via metal-ion coordination by naturally occurring surface histidines.

Quantitative long-term imaging of the functional representation of a whisker in rat barrel cortex.

GRIP1, a novel mouse protein that serves as a transcriptional coactivator in yeast for the hormone binding domains of steroid receptors.

Role of the regulatory subunit of bovine pyruvate dehydrogenase phosphatase.

Hemodynamic effects of epidermal growth factor in conscious rats and monkeys.

Electrical microstimulation suggests two different forms of representation of head-centered space in the intraparietal sulcus of rhesus monkeys.

An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteocalcin gene.

Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors.

Exchanges are not equally able to enhance meiotic chromosome segregation in yeast.

How optimization of potential functions affects protein folding.

Induction of alloantigen-specific tolerance by B cells from CD40-deficient mice.

Escherichia coli exhibits negative chemotaxis in gradients of hydrogen peroxide, hypochlorite, and N-chlorotaurine: products of the respiratory burst of phagocytic cells.

Mutagenesis and gene identification in Dictyostelium by shotgun antisense.

Identification of functional domains and evolution of Tc1-like transposable elements.

Functional complementation of xeroderma pigmentosum complementation group E by replication protein A in an in vitro system.

Epitope-specific tolerance induction with an engineered immunoglobulin.

Loss of tramtrack gene activity results in ectopic R7 cell formation, even in a sina mutant background.

Functional expression of the Schizosaccharomyces pombe Na+/H+ antiporter gene, sod2, in Saccharomyces cerevisiae.

Expansion of polyglutamine repeat in huntingtin leads to abnormal protein interactions involving calmodulin.

Exploring the active site of chorismate mutase by combinatorial mutagenesis and selection: the importance of electrostatic catalysis.

Low ethanol concentrations enhance GABAergic inhibitory postsynaptic potentials in hippocampal pyramidal neurons only after block of GABAB receptors.

Two different but related mechanisms are used in plants for the repair of genomic double-strand breaks by homologous recombination.

A synthetic random basic copolymer with promiscuous binding to class II major histocompatibility complex molecules inhibits T-cell proliferative responses to major and minor histocompatibility antigens in vitro and confers the capacity to prevent murine graft-versus-host disease in vivo.

Spatial localization of calcium channels in giant fiber lobe neurons of the squid (Loligo opalescens).

Glucocorticoid and progestin receptors are differently involved in the cooperation with a structural element of the mouse mammary tumor virus promoter.

The impact of interindividual variation in NAT2 activity on benzidine urinary metabolites and urothelial DNA adducts in exposed workers.

An increased risk of Crohn's disease in individuals who inherit the HLA class II DRB3*0301 allele.

Ozone-induced responses in Arabidopsis thaliana: the role of salicylic acid in the accumulation of defense-related transcripts and induced resistance.

A yeast manganese transporter related to the macrophage protein involved in conferring resistance to mycobacteria.

The cloned rat pancreatic polypeptide receptor exhibits profound differences to the orthologous receptor.

Oxidative stress is involved in heat-induced cell death in Saccharomyces cerevisiae.

Ancient single origin for Malagasy primates.

The C-proteinase that processes procollagens to fibrillar collagens is identical to the protein previously identified as bone morphogenic protein-1.

Mitochondrial diversity and the origins of African and European cattle.

Human V delta 2+ gamma delta T-cell tolerance to foreign antigens of Toxoplasma gondii.

Preferential induction of a Th1 immune response and inhibition of specific IgE antibody formation by plasmid DNA immunization.

Fluorescent light-induced chromatid breaks distinguish Alzheimer disease cells from normal cells in tissue culture.

In its active form, the GTP-binding protein rab8 interacts with a stress-activated protein kinase.

Inducible expression of an antibiotic peptide gene in lipopolysaccharide-challenged tracheal epithelial cells.

Oscillations in KATP channel activity promote oscillations in cytoplasmic free Ca2+ concentration in the pancreatic beta cell.

Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter.

Year: 1996 Volume: 93 Issue: 9

Year: 1996 Volume: 93 Issue: 8

Year: 1996 Volume: 93 Issue: 7

Year: 1996 Volume: 93 Issue: 6

Year: 1996 Volume: 93 Issue: 5

Year: 1996 Volume: 93 Issue: 4

Year: 1996 Volume: 93 Issue: 3

Year: 1996 Volume: 93 Issue: 2

Year: 1996 Volume: 93 Issue: 1

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Structure and function in rhodopsin: correct folding and misfolding in point mutants at and in proximity to the site of the retinitis pigmentosa mutation Leu-125-->Arg in the transmembrane helix C.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Khorana, H. G. Liu, X. Garriga, P.
Description	Author Affiliation: Garriga P ( Department of Biology, Massachusetts Institute ofTechnology, Cambridge, 02139, USA.);
Abstract	L125R is a mutation in the transmembrane helix C of rhodopsin that is associated with autosomal dominant retinitis pigmentosa. To probe the orientation of the helix and its packing in the transmembrane domain, we have prepared and studied the mutations E122R, I123R, A124R, S127R, L125F, and L125A at, and in proximity to, the above mutation site. Like L125R, the opsin expressed in COS-1 cells from E122R did not bind 11-cis-retinal, whereas those from I123R and S127R formed the rhodopsin chromophore partially. A124R opsin formed the rhodopsin chromophore (lambda max 495 nm) in the dark, but the metarhodopsin II formed on illumination decayed about 6.5 times faster than that of the wild type and was defective in transducin activation. The mutant opsins from L125F and L125A bound 11-cis-retinal only partially, and in both cases, the mixtures of the proteins produced were separated into retinal-binding and non-retinal-binding (misfolded) fractions. The purified mutant rhodopsin from L125F showed lambda max at 500 nm, whereas that from L125A showed lambda max at 503 nm. The mutant rhodopsin L125F showed abnormal bleaching behavior and both mutants on illumination showed destabilized metarhodopsin II species and reduced transducin activation. Because previous results have indicated that misfolding in rhodopsin is due to the formation of a disulfide bond other than the normal disulfide bond between Cys-110 and Cys-187 in the intradiscal domain, we conclude from the misfolding in mutants L125F and L125A that the folding in vivo in the transmembrane domain is coupled to that in the intradiscal domain.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	10
Volume Number	93
Language	English
Publisher	National Academy of Sciences
Publisher Date	1996-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Point Mutation Retinitis Pigmentosa Genetics Rhodopsin Chemistry Amino Acid Sequence Animals Arginine Binding Sites Cell Line Conserved Sequence Gene Expression Genes, Dominant Molecular Sequence Data Protein Folding Protein Structure, Secondary Metabolism Transfection Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in