NDLI: Animal fatty acid synthase: functional mapping and cloning and expression of the domain I constituent activities.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1997 Volume: 94 Issue: 26

Year: 1997 Volume: 94 Issue: 25

Year: 1997 Volume: 94 Issue: 24

Year: 1997 Volume: 94 Issue: 23

Year: 1997 Volume: 94 Issue: 22

Year: 1997 Volume: 94 Issue: 21

Year: 1997 Volume: 94 Issue: 20

Year: 1997 Volume: 94 Issue: 19

Year: 1997 Volume: 94 Issue: 18

Year: 1997 Volume: 94 Issue: 17

Year: 1997 Volume: 94 Issue: 16

Year: 1997 Volume: 94 Issue: 15

Year: 1997 Volume: 94 Issue: 14

Year: 1997 Volume: 94 Issue: 13

Year: 1997 Volume: 94 Issue: 12

Year: 1997 Volume: 94 Issue: 11

Discussion of 'The evaluation of forensic DNA evidence'.

Bacteria gone native vs. bacteria gone awry?: plasmidic transfer and bacterial evolution.

Creeping walls, softening fruit, and penetrating pollen tubes: the growing roles of expansins.

Molecular linguistics: extracting information from gene and protein sequences.

Transport bicycles.

The bi-loop, a new general four-stranded DNA motif.

Alternative splicing of the human diacylglycerol kinase zeta gene in muscle.

Design of polydactyl zinc-finger proteins for unique addressing within complex genomes.

Early embryonic lethality caused by targeted disruption of the mouse selenocysteine tRNA gene (Trsp).

Protein folding: how the mechanism of GroEL action is defined by kinetics.

A molecular mechanism for energy coupling in a membrane transport protein, the lactose permease of Escherichia coli.

Host regulation of lysogenic decision in bacteriophage lambda: transmembrane modulation of FtsH (HflB), the cII degrading protease, by HflKC (HflA).

Copper-mediated repression of the activation domain in the yeast Mac1p transcription factor.

Skn-1: evidence for a bipartite recognition helix in DNA binding.

Construction of a catalytically active iron superoxide dismutase by rational protein design.

Characterization of the thyroid Na+/I- symporter with an anti-COOH terminus antibody.

Both an N-terminal 65-kDa domain and a C-terminal 30-kDa domain of SecA cycle into the membrane at SecYEG during translocation.

I2B is a small cytosolic protein that participates in vacuole fusion.

Animal fatty acid synthase: functional mapping and cloning and expression of the domain I constituent activities.

A molecular biology-based approach to resolve the subunit orientation of lipoprotein lipase.

Activation of mitogen-activating protein kinase by glucose is not required for insulin secretion.

BRCA1 is a component of the RNA polymerase II holoenzyme.

Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast.

Synergy between adjacent zinc fingers in sequence-specific DNA recognition.

Diffusion control in an elementary protein folding reaction.

Messenger RNA deadenylylation precedes decapping in mammalian cells.

Estimation of the DNA sequence discriminatory ability of hairpin-linked lexitropsins.

The RNA-binding protein, TB-RBP, is the mouse homologue of translin, a recombination protein associated with chromosomal translocations.

Axial rotation of sliding actin filaments revealed by single-fluorophore imaging.

Femtosecond time resolution in x-ray diffraction experiments.

Conditional activation defect of a human Gsalpha mutant.

Characterization of VPS41, a gene required for vacuolar trafficking and high-affinity iron transport in yeast.

Activation of hypoxia-inducible factor 1alpha: posttranscriptional regulation and conformational change by recruitment of the Arnt transcription factor.

Expression of maspin in prostate cells is regulated by a positive ets element and a negative hormonal responsive element site recognized by androgen receptor.

The I/LWEQ module: a conserved sequence that signifies F-actin binding in functionally diverse proteins from yeast to mammals.

In situ molecular association of dystrophin with actin revealed by sensitized emission immuno-resonance energy transfer.

Developmental parameters of cell death in the wing disc of Drosophila.

Hematopoiesis in the fetal liver is impaired by targeted mutagenesis of a gene encoding a non-DNA binding subunit of the transcription factor, polyomavirus enhancer binding protein 2/core binding factor.

Cooperation of Pax2 and Pax5 in midbrain and cerebellum development.

The origin and efficient derivation of embryonic stem cells in the mouse.

Safety in numbers: sophisticated vigilance by Allenby's gerbil.

Old World fruitbat phylogeny: evidence for convergent evolution and an endemic African clade.

Molecular evolution of angiosperm mitochondrial introns and exons.

BCL8, a novel gene involved in translocations affecting band 15q11-13 in diffuse large-cell lymphoma.

SOS factors involved in translesion synthesis.

Independent signals regulate development of primary and secondary follicle structure in spleen and mesenteric lymph node.

Characterization of the major histocompatibility complex class II binding site on LAG-3 protein.

Radiation and stress-induced apoptosis: a role for Fas/Fas ligand interactions.

A lymphocyte-activating monoclonal antibody induces regression of human tumors in severe combined immunodeficient mice

The T cell receptor repertoire of intestinal intraepithelial gammadelta T lymphocytes is influenced by genes linked to the major histocompatibility complex and to the T cell receptor loci.

Elevated levels of cysteine protease activity in saliva and salivary glands of the nonobese diabetic (NOD) mouse model for Sjögren syndrome.

In the absence of the invariant chain, HLA-DR molecules display a distinct array of peptides which is influenced by the presence or absence of HLA-DM.

Differential ability of T cell subsets to undergo activation-induced cell death.

Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HIV-1.

Major histocompatibility complex gene mapping in the amphibian Xenopus implies a primordial organization.

Resource competition as a mechanism for B cell homeostasis.

Reactivation of silenced, virally transduced genes by inhibitors of histone deacetylase.

Stable gene transfer and expression of human blood coagulation factor IX after intramuscular injection of recombinant adeno-associated virus.

Growth hormone-releasing hormone antagonist MZ-4-71 inhibits in vivo proliferation of Caki-I renal adenocarcinoma.

Keratinocyte expression of the type 2 interleukin 1 receptor mediates local and specific inhibition of interleukin 1-mediated inflammation.

Human BRCA1 inhibits growth in yeast: potential use in diagnostic testing.

Blocking activator protein-1 activity, but not activating retinoic acid response element, is required for the antitumor promotion effect of retinoic acid.

Positionally cloned human disease genes: Patterns of evolutionary conservation and functional motifs

Amphotropic murine leukemia viruses induce spongiform encephalomyelopathy.

Apoptin induces apoptosis in human transformed and malignant cells but not in normal cells.

Localized gene action controlling intestinal neoplasia in mice.

Suppression of insulin-like growth factor type I receptor by a triple-helix strategy inhibits IGF-I transcription and tumorigenic potential of rat C6 glioblastoma cells.

Fas ligand deficiency in HIV disease.

Homodimerization of the human interleukin 4 receptor alpha chain induces Cepsilon germline transcripts in B cells in the absence of the interleukin 2 receptor gamma chain.

Effect of estrogen upon cyclic ADP ribose metabolism: β-Estradiol stimulates ADP ribosyl cyclase in rat uterus

Loss of functional cell surface transforming growth factor beta (TGF-beta) type 1 receptor correlates with insensitivity to TGF-beta in chronic lymphocytic leukemia.

The second finger of Urbs1 is required for iron-mediated repression of sid1 in Ustilago maydis.

Long-term potentiation involves increases in the probability of neurotransmitter release.

Calmodulin regulation of light adaptation and store-operated dark current in Drosophila photoreceptors

Early divergence of magnocellular and parvocellular functional subsystems in the embryonic primate visual system.

The neuron-restrictive silencer element: a dual enhancer/silencer crucial for patterned expression of a nicotinic receptor gene in the brain.

Identification of chemical synapses in the pharynx of Caenorhabditis elegans.

Neurotrophin regulation of ionic currents and cell size depends on cell context

Neurotoxicity associated with dual actions of homocysteine at the N-methyl-d-aspartate receptor

Selective enhancement of emotional, but not motor, learning in monoamine oxidase A-deficient mice.

Neuregulins stimulate the functional expression of $Ca^{2+}-activated$ $K^{+}$ channels in developing chicken parasympathetic neurons

Serotonin and aggressive motivation in crustaceans: altering the decision to retreat.

Na,K-ATPase subunit isoforms in human reticulocytes: evidence from reverse transcription-PCR for the presence of alpha1, alpha3, beta2, beta3, and gamma.

Novel isoforms of the beta and gamma subunits of the Xenopus epithelial Na channel provide information about the amiloride binding site and extracellular sodium sensing.

Expression of a divergent expansin gene is fruit-specific and ripening-regulated.

Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein

Complete nucleotide sequence of the chloroplast genome from the green alga Chlorella vulgaris: the existence of genes possibly involved in chloroplast division.

Event-related brain potential correlates of two states of conscious awareness in memory.

The evaluation of forensic DNA evidence. Excerpt from the Executive Summary of the National Research Council Report.

Year: 1997 Volume: 94 Issue: 10

Year: 1997 Volume: 94 Issue: 9

Year: 1997 Volume: 94 Issue: 8

Year: 1997 Volume: 94 Issue: 7

Year: 1997 Volume: 94 Issue: 6

Year: 1997 Volume: 94 Issue: 5

Year: 1997 Volume: 94 Issue: 4

Year: 1997 Volume: 94 Issue: 3

Year: 1997 Volume: 94 Issue: 2

Year: 1997 Volume: 94 Issue: 1

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Animal fatty acid synthase: functional mapping and cloning and expression of the domain I constituent activities.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Huang, W. Y. Sakai, K. Wakil, S. J. Jayakumar, A. Chirala, S. S.
Description	Author Affiliation: Chirala SS ( Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.);
Abstract	Animal fatty acid synthase (FAS; EC 2.3.1.85) is a homodimer of a multifunctional subunit protein and catalyzes the synthesis of palmitate from acetyl-CoA, malonyl-CoA, and NADPH. The subunit (Mr approximately 270,000) carries seven distinct component activities and a site for the prosthetic group 4'-phosphopantetheine (acyl carrier protein). Based on proteolytic mapping, the organization of the activity domains along the subunit polypeptide from the N terminus is as follows: beta-ketoacyl synthase, acetyl and malonyl transacylases, beta-hydroxyacyl dehydratase, enoyl reductase, beta-ketoacyl reductase, acyl carrier protein, and thioesterase. By comparing the amino acid sequences of the chicken, rat, and human synthases, we found that kallikrein cleavage sites occur in the least conserved regions of the FAS polypeptide subunit. Determining the amino acid sequences of the N-terminal end of the major kallikrein cleavage peptides helped delineate the most likely boundaries of the component activities in the cDNA-derived amino acid sequence. To confirm this organization, we cloned the chicken FAS cDNA coding for domain I and expressed it in Escherichia coli as a maltose-binding fusion protein. The isolated recombinant protein contained the activities of the acetyl and malonyl transacylases and the beta-hydroxyacyl dehydratase. Based on the boundaries of the acetyl and malonyl transacylases and the beta-hydroxyacyl dehydratase, we also cloned the appropriate cDNA fragments encoding the domains that contain the transacylases and the dehydratase in pET vectors and expressed them in E. coli as thioredoxin-6xHis fusion proteins. The purified recombinant proteins contained, respectively, the activities of the acetyl and malonyl transacylases and the dehydratase. These results not only confirmed the order of the component activities in domain I, but also paved the way for successful expression and characterization of the remaining activities.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	11
Volume Number	94
Language	English
Publisher	National Academy of Sciences
Publisher Date	1997-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Fatty Acid Synthases Biosynthesis Amino Acid Sequence Animals Cloning, Molecular Conserved Sequence Escherichia Coli Genetics Isolation & Purification Kallikreins Macromolecular Substances Molecular Weight Open Reading Frames Peptide Fragments Chemistry Peptide Mapping Recombinant Fusion Proteins Sequence Homology, Amino Acid Comparative Study Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in