NDLI: Escherichia coli NusA is required for efficient RNA binding by phage HK022 Nun protein

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Escherichia coli NusA is required for efficient RNA binding by phage HK022 Nun protein

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Escherichia coli NusA is required for efficient RNA binding by phage HK022 Nun protein

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gottesman, M. E. Watnick, R. S.
Description	Author Affiliation: Watnick RS ( Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.);
Abstract	The Nun protein of phage HK022 is an RNA binding protein of the arginine-rich motif family. Nun binds the phage lambda boxB RNA sequence (BOXB) on nascent lambda transcripts and arrests transcription elongation. Binding to BOXB is inhibited by Zn2+ and stimulated by the Escherichia coli NusA protein. Deletion of the Nun C-terminal region enhances BOXB binding and makes it independent of Zn2+ and NusA. The C terminus of Nun thus appears to interfere with the N-terminal RNA binding motif. NusA relieves this interference by binding to the Nun C terminus and forming a complex with Nun and BOXB. However, NusA also inhibits transcription arrest in vitro, in the absence of the other Nus factors. Nun deleted for its C terminus fails to bind RNA polymerase (RNAP) (RNAP) or NusA in vitro or to arrest transcription in vivo or in vitro. Our findings are consistent with the idea that NusA inhibits transcription arrest by binding to the Nun C terminus, thus blocking the interaction between Nun and RNAP. NusG, NusB, and NusE factors restore transcription arrest, presumably by promoting transfer of Nun from NusA to RNAP.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	4
Volume Number	95
Language	English
Publisher	National Academy of Sciences
Publisher Date	1998-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bacterial Proteins Metabolism Bacteriophage Lambda Genetics Coliphages Escherichia Coli Gene Expression Regulation, Viral Peptide Elongation Factors RNA-Binding Proteins Transcription Factors Viral Proteins Amino Acid Sequence Binding Sites DNA-Directed RNA Polymerases Escherichia Coli Proteins Molecular Sequence Data Protein Binding RNA, Viral Transcription, Genetic Transcriptional Elongation Factors Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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