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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Macrobbie, E. A. Lemtiri-chlieh, F. Brearley, C. A. |
| Description | Author Affiliation: Lemtiri-Chlieh F ( Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, United Kingdom.); |
| Abstract | (RS)-2-cis, 4-trans-abscisic acid (ABA), a naturally occurring plant stress hormone, elicited rapid agonist-specific changes in myo-inositol hexakisphosphate (InsP(6)) measured in intact guard cells of Solanum tuberosum (n = 5); these changes were not reproduced by (RS)-2-trans, 4-trans-abscisic acid, an inactive stereoisomer of ABA (n = 4). The electrophysiological effects of InsP(6) were assessed on both S. tuberosum (n = 14) and Vicia faba (n = 6) guard cell protoplasts. In both species, submicromolar concentrations of InsP(6), delivered through the patch electrode, mimicked the inhibitory effects of ABA and internal calcium (Ca(i)(2+)) on the inward rectifying K(+) current, I(K,in), in a dose-dependent manner. Steady state block of I(K,in) by InsP(6) was reached much more quickly in Vicia (3 min at approximately 1 microM) than Solanum (20-30 min). The effects of InsP(6) on I(K,in) were specific to the myo-inositol isomer and were not elicited by other conformers of InsP(6) (e.g., scyllo- or neo-). Chelation of Ca(2+) by inclusion of 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid or EGTA in the patch pipette together with InsP(6) prevented the inhibition of I(K,in), suggesting that the effect is Ca(2+) dependent. InsP(6) was approximately 100-fold more potent than Ins(1,4,5)P(3) in modulating I(K,in). Thus ABA increases InsP(6) in guard cells, and InsP(6) is a potent Ca(2+)-dependent inhibitor of I(K,in). Taken together, these results suggest that InsP(6) may play a major role in the physiological response of guard cells to ABA. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 15 |
| Volume Number | 97 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2000-08-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Phytic Acid Metabolism Potassium Channels, Inwardly Rectifying Potassium Channels Physiology Chelating Agents Pharmacology Egtazic Acid Analogs & Derivatives Inositol 1,4,5-Trisphosphate Plant Proteins Protoplasts Solanum Tuberosum Drug Effects Stereoisomerism Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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