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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Masucci, M. G. Heessen, S. Dantuma, N. P. Jellne, M. Lindsten, K. |
| Description | Author Affiliation: Dantuma NP ( Microbiology and Tumor Biology Center, Karolinska Institute, S-171 77 Stockholm, Sweden.); |
| Abstract | The Gly-Ala repeat (GAr) of the Epstein-Barr virus nuclear antigen-1 is a transferable element that inhibits in cis ubiquitin/proteasome-dependent proteolysis. We have investigated this inhibitory activity by using green fluorescent protein-based reporters that have been targeted for proteolysis by N end rule or ubiquitin-fusion degradation signals, resulting in various degrees of destabilization. Degradation of the green fluorescent protein substrates was inhibited on insertion of a 25-aa GAr, but strongly destabilized reporters were protected only partially. Protection could be enhanced by increasing the length of the repeat. However, reporters containing the Ub-R and ubiquitin-fusion degradation signals were degraded even in the presence of a 239-aa GAr. In accordance, insertion of a powerful degradation signal relieved the blockade of proteasomal degradation in Epstein-Barr virus nuclear antigen-1. Our findings suggest that the turnover of natural substrates may be finely tuned by GAr-like sequences that counteract targeting signals for proteasomal destruction. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 15 |
| Volume Number | 97 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2000-08-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Alanine Metabolism Cysteine Endopeptidases Epstein-Barr Virus Nuclear Antigens Glycine Herpesvirus 4, Human Multienzyme Complexes Repetitive Sequences, Amino Acid Genetics Genes, Reporter Green Fluorescent Proteins HeLa Cells Luminescent Proteins Proteasome Endopeptidase Complex Ubiquitins Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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