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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Leers-sucheta, S. Nomoto, A. Reaven, E. Azhar, S. |
| Description | Author Affiliation: Reaven E ( Geriatric Research, Education, and Clinical Center, Department of Veterans Affairs, Palo Alto Health Care System, Palo Alto, CA 94304, USA. ereaven@ispchannel.com); |
| Abstract | In the 'selective' cholesteryl ester (CE) uptake process, surface-associated lipoproteins [high density lipoprotein (HDL) and low density lipoprotein] are trapped in the space formed between closely apposed surface microvilli (microvillar channels) in hormone-stimulated steroidogenic cells. This is the same location where an HDL receptor (SR-BI) is found. In the current study, we sought to understand the relationship between SR-BI and selective CE uptake in a heterologous insect cell system. Sf9 (Spodoptera frugiperda) cells overexpressing recombinant SR-BI were examined for (i) SR-BI protein by Western blot analysis and light or electron immunomicroscopy, and (ii) selective lipoprotein CE uptake by the use of radiolabeled or fluorescent (BODIPY-CE)-labeled HDL. Noninfected or infected control Sf9 cells do not express SR-BI, show microvillar channels, or internalize CEs. An unexpected finding was the induction of a complex channel system in Sf9 cells expressing SR-BI. SR-BI-expressing cells showed many cell surface double-membraned channels, immunogold SR-BI, apolipoprotein (HDL) labeling of the channels, and high levels of selective HDL-CE uptake. Thus, double-membraned channels can be induced by expression of recombinant SR-BI in a heterologous system, and these specialized structures facilitate both the binding of HDL and selective HDL-CE uptake. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 4 |
| Volume Number | 98 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2001-02-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Antigens, CD36 Biosynthesis Cholesterol Esters Metabolism Membrane Proteins Receptors, Immunologic Receptors, Lipoprotein Animals Genetics Biological Transport Blotting, Western Boron Compounds Cell Line Fluorescent Dyes Iodine Radioisotopes Isotope Labeling Lipoproteins, HDL Lipoproteins, HDL3 Microscopy, Electron Microscopy, Fluorescence Microvilli Protein Binding Receptors, Scavenger Scavenger Receptors, Class B Spodoptera Tritium Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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