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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | White, Fletcher Alvarez De La Rosa, Diego Shao, Deren Canessa, Cecilia M. Zhang, Ping |
| Description | Author Affiliation: Alvarez de la Rosa D ( Departments of Cellular and Molecular Physiology, and Neurology, Yale University School of Medicine, New Haven, CT 06520, USA.); |
| Abstract | Acid-sensitive ion channels (ASIC) are proton-gated ion channels expressed in neurons of the mammalian central and peripheral nervous systems. The functional role of these channels is still uncertain, but they have been proposed to constitute mechanoreceptors and/or nociceptors. We have raised specific antibodies for ASIC1, ASIC2, ASIC3, and ASIC4 to examine the distribution of these proteins in neurons from dorsal root ganglia (DRG) and to determine their subcellular localization. Western blot analysis demonstrates that all four ASIC proteins are expressed in DRG and sciatic nerve. Immunohistochemical experiments and functional measurements of unitary currents from the ASICs with the patch-clamp technique indicate that ASIC1 localizes to the plasma membrane of small-, medium-, and large-diameter cells, whereas ASIC2 and ASIC3 are preferentially in medium to large cells. Neurons coexpressing ASIC2 and ASIC3 form predominantly heteromeric ASIC2-3 channels. Two spliced forms, ASIC2a and ASIC2b, colocalize in the same population of DRG neurons. Within cells, the ASICs are present mainly on the plasma membrane of the soma and cellular processes. Functional studies indicate that the pH sensitivity for inactivation of ASIC1 is much higher than the one for activation; hence, increases in proton concentration will inactivate the channel. These functional properties and localization in DRG have profound implications for the putative functional roles of ASICs in the nervous system. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 4 |
| Volume Number | 99 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2002-02-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Membrane Proteins Nerve Tissue Proteins Peripheral Nervous System Metabolism Sodium Channels Biosynthesis Physiology Acid Sensing Ion Channels Alternative Splicing Animals Blotting, Western Cell Line Cloning, Molecular DNA, Complementary Electrophysiology Ganglia, Spinal Hydrogen-Ion Concentration Immunohistochemistry Neurons Oocytes Patch-Clamp Techniques Protons Sciatic Nerve Transfection Xenopus Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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