NDLI: Exclusively targeting beta-secretase to lipid rafts by GPI-anchor addition up-regulates beta-site processing of the amyloid precursor protein.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2003 Volume: 100 Issue: 26

Year: 2003 Volume: 100 Issue: 25

Year: 2003 Volume: 100 Issue: 24

Year: 2003 Volume: 100 Issue: 23

Year: 2003 Volume: 100 Issue: 22

Year: 2003 Volume: 100 Issue: 21

Year: 2003 Volume: 100 Issue: 20

How protein thermodynamics and folding mechanisms are altered by the chaperonin cage: molecular simulations.

Maize-targeted mutagenesis: A knockout resource for maize

A candidate gene for developmental dyslexia encodes a nuclear tetratricopeptide repeat domain protein dynamically regulated in brain.

Dynamics of feature binding during object-selective attention.

The discovery of the alpha-helix and beta-sheet, the principal structural features of proteins.

Inorganic polyphosphate stimulates mammalian TOR, a kinase involved in the proliferation of mammary cancer cells.

A genomewide screen in Saccharomyces cerevisiae for genes that suppress the accumulation of mutations.

Exon junction complexes mediate the enhancing effect of splicing on mRNA expression.

The presence of p53 mutations in human osteosarcomas correlates with high levels of genomic instability.

Inhibition of HIV infectivity by a natural human isolate of Lactobacillus jensenii engineered to express functional two-domain CD4.

Ultrasensitive two-color fluorescence probes for dipole potential in phospholipid membranes.

Compensatory evolution of a precursor messenger RNA secondary structure in the Drosophila melanogaster Adh gene.

Hydrolysis of RNA/DNA hybrids containing nonpolar pyrimidine isosteres defines regions essential for HIV type 1 polypurine tract selection.

Mathematical models of RNA silencing: unidirectional amplification limits accidental self-directed reactions.

Shock-wave cosmology inside a black hole.

Low monoamine oxidase B in peripheral organs in smokers.

Poxvirus DNA topoisomerase knockout mutant exhibits decreased infectivity associated with reduced early transcription.

Tumor necrosis factor alpha-converting enzyme mediates MUC5AC mucin expression in cultured human airway epithelial cells.

Mitochondrial positioning in fission yeast is driven by association with dynamic microtubules and mitotic spindle poles.

Direct observation of dipolar couplings between distant protons in weakly aligned nucleic acids.

Human cytomegalovirus UL83-coded pp65 virion protein inhibits antiviral gene expression in infected cells.

Reactive oxygen species and ethylene play a positive role in lateral root base nodulation of a semiaquatic legume.

A1 adenosine receptors mediate hypoxia-induced ventriculomegaly.

Chronic stress and obesity: a new view of 'comfort food'.

Single-nucleotide polymorphism genotyping on optical thin-film biosensor chips.

A force-based protein biochip.

Borrelia oxidative stress response regulator, BosR: A distinctive Zn-dependent transcriptional activator

Using protein design for homology detection and active site searches.

Gating and regulation of connexin 43 (Cx43) hemichannels.

A glucose sensor hiding in a family of transporters.

Cell wall arabinan is essential for guard cell function.

Molecular cloning and spatio-temporal expression of the prostaglandin transporter: a basis for the action of prostaglandins in the bovine reproductive system.

Calsequestrin determines the functional size and stability of cardiac intracellular calcium stores: Mechanism for hereditary arrhythmia.

LAX and SPA: major regulators of shoot branching in rice.

Antisense therapy targeting MDM2 oncogene in prostate cancer: Effects on proliferation, apoptosis, multiple gene expression, and chemotherapy

Measuring stem cell frequency in epidermis: a quantitative in vivo functional assay for long-term repopulating cells.

Expression of the type 2 receptor for cysteinyl leukotrienes (CysLT2R) by human mast cells: Functional distinction from CysLT1R.

Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis.

Beta-arrestin-mediated activation of MAPK by inverse agonists reveals distinct active conformations for G protein-coupled receptors.

Helix-stabilized cyclic peptides as selective inhibitors of steroid receptor-coactivator interactions.

Redirecting tyrosine kinase signaling to an apoptotic caspase pathway through chimeric adaptor proteins.

Insulin-induced phosphorylation of FKHR (Foxo1) targets to proteasomal degradation.

ASH1, a Drosophila trithorax group protein, is required for methylation of lysine 4 residues on histone H3.

Competing ligands stabilize alternate conformations of the energy coupling motif of a TonB-dependent outer membrane transporter.

A noncognate aminoacyl-tRNA synthetase that may resolve a missing link in protein evolution.

Electron microscopic evidence for nucleation and growth of 3D acetylcholine receptor microcrystals in structured lipid-detergent matrices.

Activation of ATP-sensitive $K^{+}$ $(K_{ATP})$ channels by $H_{2}O_{2}$ underlies glutamate-dependent inhibition of striatal dopamine release

Modification by docosahexaenoic acid of age-induced alterations in gene expression and molecular composition of rat brain phospholipids.

Antiangiogenic proteins require plasma fibronectin or vitronectin for in vivo activity.

Single metallic nanoparticle imaging for protein detection in cells.

Dissecting the roles of beta-catenin and cyclin D1 during mammary development and neoplasia.

Beyond the conventional description of dynamic force spectroscopy of adhesion bonds.

A model for the cooperative free energy transduction and kinetics of ATP hydrolysis by $F_{1}-ATPase$

Evolutionary origins of the vertebrate heart: Specification of the cardiac lineage in Ciona intestinalis.

The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability.

A natural mutation in the Tyk2 pseudokinase domain underlies altered susceptibility of B10.Q/J mice to infection and autoimmunity.

A nonconserved Ala401 in the yeast Rsp5 ubiquitin ligase is involved in degradation of Gap1 permease and stress-induced abnormal proteins

The origin of war: new 14C dates from ancient Mexico.

NMR structure of the forkhead-associated domain from the Arabidopsis receptor kinase-associated protein phosphatase.

Observations of a 'weekend effect' in diurnal temperature range.

Supercoiling and denaturation in Gal repressor/heat unstable nucleoid protein (HU)-mediated DNA looping.

IL-3 receptor signaling is dispensable for BCR-ABL-induced myeloproliferative disease.

Measurements of nitric oxide on the heme iron and beta-93 thiol of human hemoglobin during cycles of oxygenation and deoxygenation.

Redefining cholesterol's role in the mechanism of the cholesterol-dependent cytolysins.

Blue light regulates an auxin-induced $K^{+}-channel$ gene in the maize coleoptile

Computational design of a $Zn^{2+}$ receptor that controls bacterial gene expression

Newborn infants can organize the auditory world.

Drosophila screening model for metastasis: Semaphorin 5c is required for l(2)gl cancer phenotype.

Genetically engineered mice with an additional class of cone photoreceptors: implications for the evolution of color vision.

Sounds and silence: an optical topography study of language recognition at birth.

EZH2 is a marker of aggressive breast cancer and promotes neoplastic transformation of breast epithelial cells.

Complete genome sequence and analysis of Wolinella succinogenes.

Antigen receptor loci poised for V(D)J rearrangement are broadly associated with BRG1 and flanked by peaks of histone H3 dimethylated at lysine 4.

Calcification in atherosclerosis: bone biology and chronic inflammation at the arterial crossroads.

Evolution's cauldron: duplication, deletion, and rearrangement in the mouse and human genomes.

Insulin regulates the activity of forkhead transcription factor Hnf-3beta/Foxa-2 by Akt-mediated phosphorylation and nuclear/cytosolic localization.

Erythropoietin reduces myocardial infarction and left ventricular functional decline after coronary artery ligation in rats.

Global climate change and mammalian species diversity in U.S. national parks.

Hepatocyte turnover during resolution of a transient hepadnaviral infection.

Probe selection for high-density oligonucleotide arrays.

An enzootic transmission cycle of Lyme borreliosis spirochetes in the southeastern United States.

Seasonality, density dependence, and population cycles in Hokkaido voles.

Quinol-based cyclic antioxidant mechanism in estrogen neuroprotection.

HSP90 interacts with RAR1 and SGT1 and is essential for RPS2-mediated disease resistance in Arabidopsis

Functional characterization of a portion of the Moloney murine leukemia virus gag gene by genetic footprinting.

A novel domain in the protein kinase SOS2 mediates interaction with the protein phosphatase 2C ABI2

Interference with T cell receptor-HLA-DR interactions by Epstein-Barr virus gp42 results in reduced T helper cell recognition.

Two CD95 tumor classes with different sensitivities to antitumor drugs.

Swapping single-stranded DNA sequence specificities of relaxases from conjugative plasmids F and R100.

Variable hematopoietic graft rejection and graft-versus-host disease in MHC-matched strains of mice.

An early modern human from the Pestera cu Oase, Romania.

The evolution of early Foraminifera.

Cytosol-endoplasmic reticulum interplay by Sec61alpha translocon in polyglutamine-mediated neurotoxicity in Drosophila.

Conserved pathways within bacteria and yeast as revealed by global protein network alignment.

Akt as a mediator of cell death.

Fruit odor discrimination and sympatric host race formation in Rhagoletis.

Combinatorial interactions of serpent, lozenge, and U-shaped regulate crystal cell lineage commitment during Drosophila hematopoiesis.

Role of residual structure in the unfolded state of a thermophilic protein.

Exclusively targeting beta-secretase to lipid rafts by GPI-anchor addition up-regulates beta-site processing of the amyloid precursor protein.

Scaling and universality in continuous length combinatorial optimization.

A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions.

The p7 polypeptide of hepatitis C virus is critical for infectivity and contains functionally important genotype-specific sequences.

Natural variation in nucleolar dominance reveals the relationship between nucleolus organizer chromatin topology and rRNA gene transcription in Arabidopsis.

Embryonic stem cells can form germ cells in vitro.

CCAAT-binding factor regulates expression of the $β_{1}$ subunit of soluble guanylyl cyclase gene in the BE2 human neuroblastoma cell line

The BCSC-1 locus at chromosome 11q23-q24 is a candidate tumor suppressor gene.

Negative selection imparts peptide specificity to the mature T cell repertoire.

War and early state formation in Oaxaca, Mexico.

Chromosome rearrangements in evolution: From gene order to genome sequence and back.

Caging helps proteins fold.

The first candidate gene for dyslexia: Turning the page of a new chapter of research.

Copper, beta-amyloid, and Alzheimer's disease: tapping a sensitive connection.

Insulin's expanding control of forkheads.

Editorial expression of concern.

Year: 2003 Volume: 100 Issue: 19

Year: 2003 Volume: 100 Issue: 18

Year: 2003 Volume: 100 Issue: 17

Year: 2003 Volume: 100 Issue: 16

Year: 2003 Volume: 100 Issue: 15

Year: 2003 Volume: 100 Issue: 14

Year: 2003 Volume: 100 Issue: 13

Year: 2003 Volume: 100 Issue: 12

Year: 2003 Volume: 100 Issue: 11

Year: 2003 Volume: 100 Issue: 10

Year: 2003 Volume: 100 Issue: 9

Year: 2003 Volume: 100 Issue: 8

Year: 2003 Volume: 100 Issue: 7

Year: 2003 Volume: 100 Issue: 6

Year: 2003 Volume: 100 Issue: 5

Year: 2003 Volume: 100 Issue: 4

Year: 2003 Volume: 100 Issue: 3

Year: 2003 Volume: 100 Issue: 2

Year: 2003 Volume: 100 Issue: Suppl 2

Year: 2003 Volume: 100 Issue: 1

Year: 2003 Volume: 100 Issue: Suppl 1

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Exclusively targeting beta-secretase to lipid rafts by GPI-anchor addition up-regulates beta-site processing of the amyloid precursor protein.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cordy, Joanna M. Hooper, Nigel M. Dingwall, Colin Turner, Anthony J. Hussain, Ishrut
Description	Author Affiliation: Cordy JM ( Proteolysis Research Group, School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom.);
Abstract	beta-Secretase (BACE, Asp-2) is a transmembrane aspartic proteinase responsible for cleaving the amyloid precursor protein (APP) to generate the soluble ectodomain sAPPbeta and its C-terminal fragment CTFbeta. CTFbeta is subsequently cleaved by gamma-secretase to produce the neurotoxic/synaptotoxic amyloid-beta peptide (Abeta) that accumulates in Alzheimer's disease. Indirect evidence has suggested that amyloidogenic APP processing may preferentially occur in lipid rafts. Here, we show that relatively little wild-type BACE is found in rafts prepared from a human neuroblastoma cell line (SH-SY5Y) by using Triton X-100 as detergent. To investigate further the significance of lipid rafts in APP processing, a glycosylphosphatidylinositol (GPI) anchor has been added to BACE, replacing the transmembrane and C-terminal domains. The GPI anchor targets the enzyme exclusively to lipid raft domains. Expression of GPIBACE substantially up-regulates the secretion of both sAPPbeta and amyloid-beta peptide over levels observed from cells overexpressing wild-type BACE. This effect was reversed when the lipid rafts were disrupted by depleting cellular cholesterol levels. These results suggest that processing of APP to the amyloid-beta peptide occurs predominantly in lipid rafts and that BACE is the rate-limiting enzyme in this process. The processing of the APP695 isoform by GPI-BACE was up-regulated 20-fold compared with wild-type BACE, whereas only a 2-fold increase in the processing of APP751/770 was seen, implying a differential compartmentation of the APP isoforms. Changes in the local membrane environment during aging may facilitate the cosegregation of APP and BACE leading to increased beta-amyloid production.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	20
Volume Number	100
Language	English
Publisher	National Academy of Sciences
Publisher Date	2003-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Amyloid Beta-Protein Precursor Metabolism Endopeptidases Glycosylphosphatidylinositols Lipid Metabolism Amino Acid Sequence Amyloid Precursor Protein Secretases Aspartic Acid Endopeptidases Cholesterol Hydrolysis Molecular Sequence Data Protein Processing, Post-Translational Tumor Cells, Cultured Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in