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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ni, Aiguo Wu, Ming Jing Nakanishi, Yuka Chavala, Sai H. |
| Description | Country affiliation: United States Author Affiliation: Ni A ( Department of Ophthalmology, University of North Carolina, Chapel Hill, North Carolina 27599-7040, USA.) |
| Abstract | Induced pluripotent stem (iPS) cells are attractive for cell replacement therapy, because they overcome ethical and immune rejection issues that are associated with embryonic stem cells. iPS cells have been derived from autonomous fibroblasts at low efficiency using multiple ectopic transcription factors. Recent evidence suggests that the epigenome of donor cell sources plays an important role in the reprogramming and differentiation characteristics of iPS cells. Thus, identification of somatic cell types that are easily accessible and are more amenable for cellular reprogramming is critical for regenerative medicine applications. Here, we identify ciliary body epithelial cells (CECs) as a new cell type for iPS cell generation that has higher reprogramming efficiency compared with fibroblasts. The ciliary body is composed of epithelial cells that are located in the anterior portion of the eye at the level of the lens and is readily surgically accessible. CECs also have a reduced reprogramming requirement, as we demonstrate that ectopic Sox2 and c-Myc are dispensable. Enhanced reprogramming efficiency may be due to increased basal levels of Sox2 in CECs. In addition, we are the first to report a cellular reprogramming haploinsufficiency observed when reprogramming with fewer factors (Oct4 and Klf4) in Sox2 hemizygous cells. Taken together, endogenous Sox2 levels are critical for the enhanced efficiency and reduced exogenous requirement that permit facile cellular reprogramming of CECs. |
| File Format | HTM / HTML |
| ISSN | 15473287 |
| e-ISSN | 15578534 |
| DOI | 10.1089/scd.2012.0600 |
| Journal | Stem Cells and Development |
| Issue Number | 18 |
| Volume Number | 22 |
| Language | English |
| Publisher | Mary Ann Liebert, Inc. |
| Publisher Date | 2013-09-15 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Cell Biology Discipline Hematology Cellular Reprogramming Genetics Ciliary Body Cytology Epithelial Cells Metabolism Induced Pluripotent Stem Cells Soxb1 Transcription Factors Animals Cell Differentiation Cells, Cultured Fibroblasts Gene Knock-in Techniques Kruppel-like Transcription Factors Mice Mice, Transgenic Octamer Transcription Factor-3 Proto-oncogene Proteins C-myc Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Hematology |
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