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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Myong, Sua Kidwell, Mary Anne Koh, Hye Ran Doudna, Jennifer A. Ragunathan, Kaushik |
| Description | Author Affiliation: Koh HR ( Department of Physics, University of Illinois, Urbana, IL 61801, USA.); |
| Abstract | The proteins harboring double-stranded RNA binding domains (dsRBDs) play diverse functional roles such as RNA localization, splicing, editing, export, and translation, yet mechanistic basis and functional significance of dsRBDs remain unclear. To unravel this enigma, we investigated transactivation response RNA binding protein (TRBP) consisting of three dsRBDs, which functions in HIV replication, protein kinase R(PKR)-mediated immune response, and RNA silencing. Here we report an ATP-independent diffusion activity of TRBP exclusively on dsRNA in a length-dependent manner. The first two dsRBDs of TRBP are essential for diffusion, whereas the third dsRBD is dispensable. Two homologs of TRBP, PKR activator and R3D1-L, displayed the same diffusion, implying a universality of the diffusion activity among this protein family. Furthermore, a Dicer-TRBP complex on dsRNA exhibited dynamic diffusion, which was correlated with Dicer's catalytic activity. These results implicate the dsRNA-specific diffusion activity of TRBP that contributes to enhancing siRNA and miRNA processing by Dicer. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 1 |
| Volume Number | 110 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2013-01-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Drosophila Proteins Metabolism Multiprotein Complexes RNA, Double-Stranded RNA-Binding Proteins Ribonuclease III Adenosine Triphosphate Animals Diffusion Electrophoresis, Polyacrylamide Gel Fluorescence Resonance Energy Transfer HeLa Cells Microscopy, Fluorescence Oligonucleotides Genetics Protein Structure, Tertiary Physiology Protein Transport RNA Interference Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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