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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Purcell, Robert H. Bidzhieva, Bella Kouiavskaia, Diana Chumakov, Konstantin Fischer, Elizabeth R. Chen, Zhaochun Hansen, Bryan T. Ludtke, Steven J. Makiya, Michelle Agulto, Liane |
| Description | Author Affiliation: Chen Z ( Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.); |
| Abstract | Most structural information about poliovirus interaction with neutralizing antibodies was obtained in the 1980s in studies of mouse monoclonal antibodies. Recently we have isolated a number of human/chimpanzee anti-poliovirus antibodies and demonstrated that one of them, MAb A12, could neutralize polioviruses of both serotypes 1 and 2. This communication presents data on isolation of an additional cross-neutralizing antibody (F12) and identification of a previously unknown epitope on the surface of poliovirus virions. Epitope mapping was performed by sequencing of antibody-resistant mutants and by cryo-EM of complexes of virions with Fab fragments. The results have demonstrated that both cross-neutralizing antibodies bind the site located at the bottom of the canyon surrounding the fivefold axis of symmetry that was previously shown to interact with cellular poliovirus receptor CD155. However, the same antibody binds to serotypes 1 and 2 through different specific interactions. It was also shown to interact with type 3 poliovirus, albeit with about 10-fold lower affinity, insufficient for effective neutralization. Antibody interaction with the binding site of the cellular receptor may explain its broad reactivity and suggest that further screening or antibody engineering could lead to a universal antibody capable of neutralizing all three serotypes of poliovirus. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 50 |
| Volume Number | 110 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2013-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Antibodies, Viral Immunology Capsid Metabolism Cross Reactions Models, Molecular Poliovirus Antibody Specificity Chemistry Cell Surface Display Techniques Cryoelectron Microscopy Disease Eradication Enzyme-Linked Immunosorbent Assay HEK293 Cells Molecular Sequence Data Neutralization Tests Sequence Analysis, DNA Species Specificity Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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