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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Nolf, Jonah Van De Slijke, Eveline Heijde, Marc Yin, Ruohe Ulm, Roman Binkert, Melanie Rizzini, Luca Persiau, Geert De Jaeger, Geert Gevaert, Kris Ares-orpel, Florence |
| Description | Author Affiliation: Heijde M ( Department of Botany and Plant Biology, Sciences III, University of Geneva, CH-1211 Geneva 4, Switzerland.); |
| Abstract | Arabidopsis thaliana UV RESISTANCE LOCUS 8 (UVR8) is a UV-B photoreceptor that initiates photomorphogenic responses underlying acclimation and UV-B tolerance in plants. UVR8 is a homodimer in its ground state, and UV-B exposure results in its instantaneous monomerization followed by interaction with CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), a major factor in UV-B signaling. UV-B photoreception by UVR8 is based on intrinsic tryptophan aromatic amino acid residues, with tryptophan-285 as the main chromophore. We generated transgenic plants expressing UVR8 with a single amino acid change of tryptophan-285 to alanine. $UVR8^{W285A}$ appears monomeric and shows UV-B–independent interaction with COP1. Phenotypically, the plants expressing $UVR8^{W285A}$ exhibit constitutive photomorphogenesis associated with constitutive activation of target genes, elevated levels of anthocyanins, and enhanced, acclimation-independent UV-B tolerance. Moreover, we have identified COP1, REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 and 2 (RUP1 and RUP2), and the SUPPRESSOR OF PHYA-105 (SPA) family as proteins copurifying with $UVR8^{W285A}.$ Whereas COP1, RUP1, and RUP2 are known to directly interact with UVR8, we show that SPA1 interacts with UVR8 indirectly through COP1. We conclude that $UVR8^{W285A}$ is a constitutively active UVR8 photoreceptor variant in Arabidopsis, as is consistent with the crucial importance of monomer formation and COP1 binding for UVR8 activity. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 50 |
| Volume Number | 110 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2013-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Arabidopsis Proteins Genetics Arabidopsis Chromosomal Proteins, Non-Histone Phenotype Photoreceptors, Plant Anthocyanins Metabolism Cell Cycle Proteins Chromatography, Gel Chromatography, Liquid Electrophoresis, Polyacrylamide Gel Genetic Engineering Immunoprecipitation Mutation, Missense Plants, Genetically Modified Real-Time Polymerase Chain Reaction Tandem Mass Spectrometry Two-Hybrid System Techniques Ubiquitin-Protein Ligases Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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