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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ensenauer, R. Hartl, D. Vockley, J. Roscher, A. A. Fuchs, U. |
| Description | Country affiliation: Germany Author Affiliation: Ensenauer R ( Children's Research Center, Dr. von Hauner Children's Hospital, Ludwig-Maximilians-Universität München, Munich, Germany. regina.ensenauer@med.uni-muenchen.de) |
| Abstract | Magnetic force combined with magnetic nanoparticles recently has shown potential for enhancing nucleic acid delivery. Achieving effective siRNA delivery into primary cultured cells is challenging. We compared the utility of magnetofection with lipofection procedures for siRNA delivery to primary and immortalized mammalian fibroblasts. Transfection efficiency and cell viability were analyzed by flow cytometry and effects of gene knockdown were quantified by real-time PCR. Lipofectamine 2000 and magnetofection achieved high transfection efficiencies comparable to similar gene silencing effects of about 80%; the cytotoxic effect of magnetofection, however, was significantly less. Magnetofection is a reliable and gentle alternative method with low cytotoxicity for siRNA delivery into difficult to transfect cells such as mammalian fibroblasts. These features are especially advantageous for functional end point analyses of gene silencing, e.g., on the metabolite level. |
| File Format | HTM / HTML |
| ISSN | 10520295 |
| e-ISSN | 14737760 |
| DOI | 10.3109/10520291003675485 |
| Journal | Biotechnic & Histochemistry |
| Issue Number | 4 |
| Volume Number | 86 |
| Language | English |
| Publisher | Taylor & Francis |
| Publisher Date | 2011-08-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Discipline Technology Discipline Histocytochemistry Magnetite Nanoparticles Administration & Dosage Rna, Messenger Metabolism Rna, Small Interfering Genetics Transfection Animals Cell Culture Techniques Cell Line, Tumor Cell Survival Fibroblasts Gene Silencing Lipids Chemistry Mice Rna Interference Physiology Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Histology Medical Laboratory Technology |
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