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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Kirshenbaum, Kent Dehan, Elinor Ouerfelli, Ouathek Piano, Fabio Utku, Yeliz Zuckermann, Ronald N. Pagano, Michele |
| Copyright Year | 2006 |
| Abstract | RNA interference (RNAi) techniques hold forth great promise for therapeutic silencing of deleterious genes. However, clinical applications of RNAi require the development of safe and efficient methods for intracellular delivery of small interfering RNA (siRNA) oligonucleotides specific to targeted genes. We describe the use of a lipitoid, a cationic oligopeptoid–phospholipid conjugate, for non-viral transfection of synthetic siRNA oligos in cell culture. This peptidomimetic delivery vehicle allows for efficient siRNA transfection in a variety of human cell lines with negligible toxicity and promotes extensive downregulation of the targeted genes at both the protein and the mRNA level. We compare the lipitoid reagent to a standard commercial transfection reagent. The lipitoid is highly efficient even in primary IMR-90 human lung fibroblasts in which other commercial reagents are typically ineffective. |
| Starting Page | 312 |
| Ending Page | 317 |
| Page Count | 6 |
| File Format | HTM / HTML PDF |
| ISSN | 1742206X |
| Volume Number | 2 |
| Issue Number | 6-7 |
| Journal | Molecular BioSystems |
| DOI | 10.1039/b603229j |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | Cell culture RNA interference Transfection Messenger RNA Delivery (commerce) Peptidomimetic Lung Small interfering RNA Reagent Protein |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology Biotechnology |
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