NDLI: An Electrochemical Biosensor Based on a Myoglobin-specific Binding Peptide for Early Diagnosis of Acute Myocardial Infarction.

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An Electrochemical Biosensor Based on a Myoglobin-specific Binding Peptide for Early Diagnosis of Acute Myocardial Infarction.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lee, Hyeong Yun Choi, Ji Suk Guruprasath, Padmanaban Lee, Byung-Heon Cho, Yong Woo
Description	Author Affiliation: Lee HY ( Department of Chemical Engineering, Hanyang University.)
Abstract	In this study, a simple, highly sensitive electrochemical biosensor for myoglobin was developed using a myoglobin-specific binding peptide as a sensing probe. A peptide (Myo-3R7, CPSTLGASC, 838 Da) identified by phage display and that specifically binds to myoglobin was covalently immobilized on a gold electrode functionalized via a dithiobis(succinimidyl propionate) (DSP) self-assembled monolayer (SAM). The peptide immobilization was confirmed with fluorescence microarray scanning and cyclic voltammetry (CV). The electrochemical performance of the biosensor with respect to myoglobin was characterized by CV and differential pulse voltammetry (DPV) using Fe(CN)6(3-)/Fe(CN)6(4-) as a redox probe. We successfully detected myoglobin in a broad working range of 17.8 to 1780 ng mL(-1) with a correlation coefficient (R(2)) of 0.998. The estimated limit of detection (LOD) was fairly low, 9.8 ng mL(-1) in 30 min. The electrochemical biosensor based on a myoglobin-specific binding peptide offers sensitivity, selectivity, and rapidity, making it an attractive tool for the early detection of cardiac infarction.
File Format	HTM / HTML
ISSN	09106340
e-ISSN	13482246
Journal	Analytical Sciences
Issue Number	7
Volume Number	31
Language	English
Publisher	The Japan Society for Analytical Chemistry
Publisher Date	2015-01-01
Publisher Place	Japan
Access Restriction	Open
Subject Keyword	Discipline Analytical Discipline Chemistry Techniques Biosensing Techniques Myocardial Infarction Diagnosis Myoglobin Metabolism Oligopeptides Acute Disease Amino Acid Sequence Early Diagnosis Electrochemistry Ferricyanides Chemistry Limit Of Detection Models, Molecular Protein Conformation Substrate Specificity Succinimides Time Factors Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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