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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zaitouna, Anita J. Maben, Alex J. Lai, Rebecca Y. |
| Description | Country affiliation: United States Author Affiliation: Zaitouna AJ ( University of Nebraska-Lincoln, 651 Hamilton Hall, Lincoln, NE 68588-0304, USA.); Maben AJ ( University of Nebraska-Lincoln, 651 Hamilton Hall, Lincoln, NE 68588-0304, USA.); Lai RY ( University of Nebraska-Lincoln, 651 Hamilton Hall, Lincoln, NE 68588-0304, USA. Electronic address: rlai2@unl.edu.) |
| Abstract | We investigated the effect of incorporating extra amino acids (AA) at the n-terminus of the thiolated and methylene blue-modified peptide probe on both specificity and selectivity of an electrochemical peptide-based (E-PB) HIV sensor. The addition of a flexible (SG)3 hexapeptide is, in particular, useful in improving sensor selectivity, whereas the addition of a highly hydrophilic (EK)3 hexapeptide has shown to be effective in enhancing sensor specificity. Overall, both E-PB sensors fabricated using peptide probes with the added AA (SG-EAA and EK-EAA) showed better specificity and selectivity, especially when compared to the sensor fabricated using a peptide probe without the extra AA (EAA). For example, the selectivity factor recorded in the 50% saliva was â¼2.5 for the EAA sensor, whereas the selectivity factor was 7.8 for both the SG-EAA and EK-EAA sensors. Other sensor properties such as the limit of detection and dynamic range were minimally affected by the addition of the six AA sequence. The limit of detection was 0.5 nM for the EAA sensor and 1 nM for both SG-EAA and EK-EAA sensors. The saturation target concentration was â¼200 nM for all three sensors. Unlike previously reported E-PB HIV sensors, the peptide probe functions as both the recognition element and antifouling passivating agent; this modification eliminates the need to include an additional antifouling diluent, which simplifies the sensor design and fabrication protocol. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 886 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-07-30 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Amino Acids Chemistry Biosensing Techniques Methods Electrochemical Techniques Hiv Isolation & Purification Peptides Saliva Virology Urine Amino Acid Sequence Hiv Infections Diagnosis Humans Methylene Blue Molecular Sequence Data Sulfhydryl Compounds Journal Article Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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