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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Derycke, Lara Stove, Christophe Vercoutter-Edouart, Anne-Sophie De Wever, Olivier Dollé, Laurent Colpaert, Nathalie Depypere, Herman Michalski, Jean-Claude Bracke, Marc |
| Description | Country affiliation: Belgium Author Affiliation: Derycke L ( Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University Hospital, De Pintelaan 185, Ghent, Belgium.) |
| Abstract | Human MCF-7/6 breast cancer cells differ from their MCF-7/AZ counterparts by their invasiveness in a number of assays in vitro, such as invasion of MCF-7 spheroids into embryonic chick heart fragments or type I collagen gels. Comparative proteomic analysis of these two variants revealed an identical pattern, except for a 230 kDa protein present in the invasive MCF-7/6 variant, but hardly detectable in the non-invasive MCF-7/AZ one. This protein appeared to be the non-muscle myosin IIA heavy chain (NMIIA), also coined MYH9. Experimental inhibition of NMIIA by reducing either its expression (via stable shRNA transduction) or its function (via the specific ATPase inhibitor blebbistatin) underpinned the decisive role of NMIIA in MCF-7 cell invasion. Inhibition of NMIIA indeed blocked the invasion of MCF-7/6 cells in three-dimensional invasion substrata such as embryonic chick heart fragments and type I collagen gels. Invasiveness of MCF-7/6 cells has been related to poor formation and compaction of aggregates, due to a functionally defective E-cadherin/catenin complex. Both genetic and pharmacological inhibition of NMIIA stimulated MCF-7/6 cell aggregation. Together, these data indicate that NMIIA is a decisive protein for MCF-7 cells to invade, indicating that this molecule is a candidate for targeted anti-invasive treatment. |
| File Format | HTM / HTML |
| ISSN | 02146282 |
| e-ISSN | 16963547 |
| Journal | The International Journal of Developmental Biology |
| Issue Number | 7-9 |
| Volume Number | 55 |
| Language | English |
| Publisher | University of the Basque Country Press |
| Publisher Date | 2011-01-01 |
| Publisher Place | Spain |
| Access Restriction | Open |
| Subject Keyword | Discipline Embryology Discipline Biology Breast Neoplasms Pathology Physiopathology Cell Aggregation Physiology Molecular Motor Proteins Myosin Heavy Chains Neoplasm Invasiveness Animals Cell Line, Tumor Chick Embryo Gene Knockdown Techniques Heterocyclic Compounds With 4 Or More Rings Pharmacology Antagonists & Inhibitors Genetics Rna, Small Interfering Spheroids, Cellular Tumor Stem Cell Assay Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Developmental Biology Embryology |
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