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Epinephrine-induced MMP expression is different between skeletal fibroblasts and myoblasts

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cha, M. C. Purslow, P. P.
Description	Country affiliation: Canada Author Affiliation: Cha MC ( Department of Food Science, University of Guelph, Guelph, Ontario, Canada.)
Abstract	Skeletal fibroblasts and myoblasts are among the cell types currently being considered in cell therapy for ischaemic heart disease. To investigate whether the expression of the tissue-remodelling proteolytic enzymes matrix metalloproteinases (MMPs) and the cellular energy regulator AMP-activated protein kinase (AMPK) is comparable between the two cell lines in response to epinephrine treatment, mouse skeletal fibroblasts (NOR-10) and myoblasts (C2C12) were treated with or without a low $(11 nmol·l^{-1})$ or high $(55 nmol·l^{-1})$ dose of epinephrine for 2 or 6 h. Cellular MMP-3 expression was increased by the high-dose epinephrine at both treatment periods in both cell lines. Cellular MMP-2 and MMP-13 expressions were amplified by the 2- or 6-h epinephrine incubation in fibroblasts. However, in myoblasts, such an increase was only seen at the longer treatment time. An elevated AMPKα expression was observed after a 2-h presence of epinephrine in both cell lines, which matches temporally with the early increased cellular MMP-2 and MMP-13 expression in fibroblasts. Activity of secreted MMP-2 increased only after 6-h epinephrine treatment in both cell types. Our data suggest that skeletal fibroblasts respond earlier to epinephrine application in terms of endogenous synthesis of the proteolytic and the energy homeostasis enzymes, whereas such response occurs later and to a milder dose of the beta adrenergic agonist in myoblasts. Copyright © 2011 John Wiley & Sons, Ltd.
File Format	HTM / HTML
ISSN	02636484
Issue Number	7
Volume Number	29
e-ISSN	10990844
Journal	Cell Biochemistry and Function
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2011-10-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Cell Biology Discipline Biochemistry Epinephrine Pharmacology Fibroblasts Drug Effects Matrix Metalloproteinases Metabolism Myoblasts Amp-activated Protein Kinases Animals Blotting, Western Cell Line Culture Media, Serum-free Enzyme Activation Enzymology Gelatinases Homeostasis Secretion Mice Microscopy, Fluorescence Time Factors Comparative Study Journal Article
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Biochemistry Clinical Biochemistry

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