NDLI: PPADS does not block contraction-induced prostaglandin $E

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	McCord, Jennifer L. Hayes, Shawn G. Kaufman, Marc P.
Description	Country affiliation: United States Author Affiliation: McCord JL ( Pennsylvania State Heart and Vascular Institute, Pennsylvania State University College of Medicine, 500 Univ. Dr., Mail Code H047, C2706, Hershey Medical Ctr., Hershey, PA 17033, USA. jmccord@hmc.psu.edu)
Abstract	Pyridoxal-phosphate-6-azophenyl-2′-4-disulfonate (PPADS), a purinergic 2 $(P_{2})$ receptor antagonist, has been shown to attenuate the exercise pressor reflex in cats. In vitro, however, PPADS has been shown to block the production of prostaglandins, some of which play a role in evoking the exercise pressor reflex. Thus the possibility exists that PPADS blocks the exercise pressor reflex through a reduction in prostaglandin synthesis rather than through the blockade of $P_{2}$ receptors. Using microdialysis, we collected interstitial fluid from skeletal muscle to determine prostaglandin $E_{2}$ $(PGE_{2})$ concentrations during the intermittent contraction of the triceps surae muscle before and after a popliteal arterial injection of PPADS (10 mg/kg). We found that the $PGE_{2}$ concentration increased in response to the intermittent contraction before and after the injection of PPADS (both, P < 0.05). PPADS reduced the pressor response to exercise (P < 0.05) but had no effect on the magnitude of $PGE_{2}$ production during contraction (P = 0.48). These experiments demonstrate that PPADS does not block the exercise pressor reflex through a reduction in $PGE_{2}$ synthesis. We suggest that $PGE_{2}$ and $P_{2}$ receptors play independent roles in stimulating the exercise pressor reflex.
File Format	HTM / HTML
ISSN	03636135
e-ISSN	15221539
DOI	10.1152/ajpheart.00904.2008
Journal	AJP: Heart and Circulatory Physiology
Issue Number	5
Volume Number	295
Language	English
Publisher	American Physiological Society
Publisher Date	2008-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physiology Discipline Cardiology Baroreflex Drug Effects Dinoprostone Biosynthesis Muscle Contraction Muscle, Skeletal Purinergic P2 Receptor Antagonists Pyridoxal Phosphate Analogs & Derivatives Animals Blood Pressure Cyclooxygenase Inhibitors Pharmacology Decerebrate State Heart Rate Injections, Intra-arterial Microdialysis Blood Supply Metabolism Popliteal Artery Administration & Dosage Receptors, Purinergic P2 Research Support, N.i.h., Extramural
Alternative Title	PPADS does not block contraction-induced prostaglandin E2 synthesis in cat skeletal muscle
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Cardiology and Cardiovascular Medicine

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PPADS does not block contraction-induced prostaglandin $E_{2}$ synthesis in cat skeletal muscle

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