NDLI: Expression of cyclophilin A in gastric adenocarcinoma patients and its inverse association with local relapses and distant metastasis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Grigoryeva, Evgeniya S. Cherdyntseva, Nadezhda V. Karbyshev, Mikhail S. Volkomorov, Viktor V. Stepanov, Ivan V. Zavyalova, Marina V. Perelmuter, Vladimir M. Buldakov, Mikhail A. Afanasjev, Sergey G. Tuzikov, Sergey A. Bukurova, Yulia A. Lisitsyn, Nikolai A. Beresten, Sergey F.
Description	Author Affiliation: Grigoryeva ES ( Cancer Research Institute of Siberian Branch of RAMS, Tomsk, Russian Federation, grigorieva@oncology.tomsk.ru.)
Abstract	The aim of this study was to identify new protein markers of the intestinal and diffuse type gastric adenocarcinoma and to determine their relation to local relapses and distant metastasis. Using two-dimensional gel electrophoresis, we searched for proteins that are overexpressed in the intestinal and/or diffuse type gastric adenocarcinoma, as compared to matched normal mucosa samples with further change confirmation by Western blot. Expression of the selected proteins was further assessed by immunohistocemistry in a large panel of gastric adenocarcinoma with various clinicopathological features. Expression level of cyclophilin A measured with western blot appeared to be increased on average ten times in 63 % of gastric adenocarcinoma vs. paired samples of normal mucosa. The frequency of immunihistochemistry detected cyclophilin A protein expression was found to be equal in tumor of both histotypes, but staining intensity was higher in intestinal versus diffuse types of gastric adenocarcinoma. cyclophilin A protein expression appeared to be lower in deeply invading glandular and cribriform structures of intestinal tumors, as well as in discretely placed groups of the intestinal tumor cells. Local relapses as well as distant metastases registered within 3 year follow up were observed to occur much less frequently in patients with positive cyclophilin A immunostaining in gastric tumors. Analysis of cyclophilin A expression has a potential value for prognosis of gastric adenocarcinoma recurrence and distant metastasis.
File Format	HTM / HTML
ISSN	12194956
Issue Number	2
Volume Number	20
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2014-04-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Adenocarcinoma Metabolism Pathology Cyclophilin A Neoplasm Metastasis Neoplasm Recurrence, Local Stomach Neoplasms Tumor Markers, Biological Female Gastric Mucosa Humans Immunohistochemistry Methods Intestinal Neoplasms Male Middle Aged Neoplasm Staging Prognosis Journal Article
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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