NDLI: Autologous dendritic cell based adoptive immunotherapy of patients with colorectal cancer-A phase I-II study.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hunyadi, János András, Csilla Szabó, Imre Szántó, János Szluha, Kornélia Sipka, Sándor Kovács, Péter Kiss, Attila Szegedi, Gyula Altorjay, István Sápy, Péter Antal-Szalmás, Péter Tóth, László Fazekas, György Rajnavölgyi, Éva
Description	Country affiliation: Hungary Author Affiliation: Hunyadi J ( Clinical Centre for Cell Therapy, Medical and Health Science Center, University of Debrecen, Nagyerdei krt. 98, 4032, Debrecen, Hungary.)
Abstract	Dendritic cell-based active immunotherapies of cancer patients are aimed to provoke the proliferation and differentiation of tumor-specific CD4(+) and CD8(+) T-lymphocytes towards protective effector cells. Isolation and in vitro differentiation of circulating blood monocytes has been established a reasonable platform for adoptively transferred DC-based immunotherapies. In the present study the safety and tolerability of vaccination by autologous tumor cell lysates (oncolysate)- or carcinoembriogenic antigen (CEA)-loaded DCs in patients with colorectal cancer was investigated in a phase I-II trial. The study included 12 patients with histologically confirmed colorectal cancer (Dukes B2-C stages). Six of the patients received oncolysate-pulsed, whereas the other six received recombinant CEA-loaded autologous DCs. The potential of the tumor antigen-loaded DCs to provoke the patient's immune system was studied both in vivo and in vitro. The clinical outcome of the therapy evaluated after 7 years revealed that none of the six patients treated with oncolysate-loaded DCs showed relapse of colorectal cancer, whereas three out of the six patients treated with CEA-loaded DCs died because of tumor relapse. Immunization with both the oncolysate- and the CEA-loaded autologous DCs induced measurable immune responses, which could be detected in vivo by cutaneous reactions and in vitro by lymphocyte proliferation assay. Our results show that vaccination by autologous DCs loaded with autologous oncolysates containing various tumor antigens represents a well tolerated therapeutic modality in patients with colorectal cancer without any detectable adverse effects. Demonstration of the efficacy of such therapy needs further studies with increased number of patients.
File Format	HTM / HTML
ISSN	12194956
Issue Number	2
Volume Number	20
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2014-04-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Autoantigens Immunology Colorectal Neoplasms Therapy Dendritic Cells Adolescent Aged Antigens, Neoplasm Cd4-positive T-lymphocytes Cd8-positive T-lymphocytes Carcinoembryonic Antigen Cell Differentiation Cell Proliferation Physiology Female Humans Immunotherapy, Adoptive Methods Male Middle Aged Neoplasm Recurrence, Local Clinical Trial, Phase I Clinical Trial, Phase Ii Journal Article
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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