NDLI: Study of enzyme activities and protein content of beluga (Huso huso) semen before and after cryopreservation.

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Study of enzyme activities and protein content of beluga (Huso huso) semen before and after cryopreservation.

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Study of enzyme activities and protein content of beluga (Huso huso) semen before and after cryopreservation.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Aramli, M. S.
Description	Country affiliation: Iran Author Affiliation: Aramli MS ( Young Researchers and Elites Club, Ardabil Branch, Islamic Azad University, Ardabil, Iran.)
Abstract	Knowledge gained regarding the biochemical processes that occur during sperm collection, processing and freezing-thawing might improve current sperm cryopreservation techniques. In our present study, we determined the effect of cryopreservation on the total protein concentration (TP) and the activities of certain enzymes in semen samples from the beluga (Huso huso). The TP content of the seminal plasma of fresh semen was 0.47 ± 0.026 g/l, and the TP after cryopreservation was 1.86 ± 0.6 g/l. The activities of acid phosphatase (0.82 ± 0.042 U/l), lactate dehydrogenase (234.4 ± 19.4 U/l), arylsulfatase (143.1 ± 32.5 U/l) and ß-N-acetylglucosaminidase (58.39 ± 4.14 U/l) in the seminal plasma of fresh semen were significantly lower than those in the supernatant of frozen-thawed semen samples (7.43 ± 0.64, 3224.6 ± 167.2, 422.6 ± 21.3 and 90.2 ± 5.37 U/l respectively). These parameters may be useful as biomarkers for estimating damage to the cell membrane of spermatozoa caused by freezing-thawing.
File Format	HTM / HTML
ISSN	09312439
Issue Number	1
Volume Number	99
e-ISSN	14390396
Journal	Journal of Animal Physiology and Animal Nutrition
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2015-02-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Physiology Discipline Veterinary Medicine Discipline Nutritional Sciences Cryopreservation Veterinary Fishes Physiology Proteins Chemistry Semen Preservation Semen Enzymology Animals Male Journal Article
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Food Animals

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