NDLI: Critical role of tyrosine 79 in the fluorescence resonance energy transfer and terbium(III)-dependent self-assembly of ciliate Euplotes octocarinatus centrin.

Please wait, while we are loading the content...

Critical role of tyrosine 79 in the fluorescence resonance energy transfer and terbium(III)-dependent self-assembly of ciliate Euplotes octocarinatus centrin.

Methionine motifs of copper transport proteins provide general and flexible thioether-only binding sites for Cu(I) and Ag(I).

Molecular and cellular mechanism of the effect of La(III) on horseradish peroxidase.

Vanadium compounds discriminate hepatoma and normal hepatic cells by differential regulation of reactive oxygen species

Molecular interaction between europium decatungstate and histone H1 and its application as a novel biological labeling agent.

The annotation of full zinc proteomes.

First structural evidence for the mode of diffusion of aromatic ligands and ligand-induced closure of the hydrophobic channel in heme peroxidases

Aromatic C-H bond hydroxylation by P450 peroxygenases: a facile colorimetric assay for monooxygenation activities of enzymes based on Russig's blue formation.

Complex formation and stability of westiellamide derivatives with copper(II).

Correlation of concentrations of selected trace elements with Gleason grade of prostate tissues.

Spectroscopic insights into axial ligation and active-site H-bonding in substrate-bound human heme oxygenase-2.

Dynamic accumulation and redistribution of methylmercury in the lens of developing zebrafish embryos and larvae.

Antiproliferative Pt(IV) complexes: synthesis, biological activity, and quantitative structure-activity relationship modeling.

Structural basis for the decrease in the outward potassium channel current induced by lanthanum

Year: 2010 Volume: 15 Issue: 6

Year: 2010 Volume: 15 Issue: 5

Year: 2010 Volume: 15 Issue: 4

Year: 2010 Volume: 15 Issue: 3

Year: 2010 Volume: 15 Issue: 2

Year: 2010 Volume: 15 Issue: 1

Year: 2009 Volume: 14

Year: 2008 Volume: 13

Year: 2007 Volume: 12

Year: 2005 Volume: 10

Critical role of tyrosine 79 in the fluorescence resonance energy transfer and terbium(III)-dependent self-assembly of ciliate Euplotes octocarinatus centrin.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Duan, Lian Liu, Wen Wang, Zhi-Jun Liang, Ai-Hua Yang, Bin-Sheng
Description	Country affiliation: China Author Affiliation: Duan L ( Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan, 030006, China.)
Abstract	Ciliate Euplotes octocarinatus centrin (EoCen) is a member of the EF-hand superfamily of calcium-binding proteins. It has been proven, using Tb3+ as a fluorescence probe, that EoCen has four calcium-binding sites. The sensitized emission arises from nonradiative energy transfer between the three tyrosine residues (Tyr46, Tyr72, and Tyr79) of the N-terminal half and the bound Tb3+ ions. To determine the most critical of the three tyrosine residues for the process of fluorescence resonance energy transfer, six mutants of the N-terminal domain of EoCen, which contain one (N-Tyr46/N-Tyr72/N-Tyr79) or two (N-Y46F/N-Y72F/N-Y79F) tyrosine residues, were obtained by site-directed mutagenesis. The aromatic residue-sensitized Tb3+ fluorescence of N-Y79F was most affected, displaying a 50% reduction compared with wild-type N-EoCen. Among the tyrosines, Tyr79 is the shortest mean distance from the protein-bound Tb3+ (at sites I/II), as calculated via the Förster mechanism. The steady-state and time-resolved fluorescence parameters of the wild-type N-EoCen and the three double mutants suggest that Tyr79, which exists in a hydrophobic environment, has the highest quantum yield and a relatively long average lifetime. The decay of Tyr79 is the least heterogeneous among the three tyrosine residues. In addition, molecular modeling shows that a critical hydrogen bond is formed between the 4-hydroxyl group of Tyr79 and the oxygen from the side chains of the residue Asn39. Kinetic experiments on tyrosine and Tb3+ fluorescence demonstrate that tyrosine fluorescence quenching is largely due to the self-assembly of EoCen, and that the quenching degrees of the mutants differ. Resonance light scattering and crosslinking analysis carried out on the full-length single mutants (Y46F, Y72F, and Y79F) showed that Tyr79 also plays the most important role in the Tb3+-dependent self-assembly of EoCen among the three tyrosines.
File Format	HTM / HTML
ISSN	09498257
Issue Number	7
Volume Number	15
e-ISSN	14321327
Journal	JBIC Journal of Biological Inorganic Chemistry
Language	English
Publisher	Springer
Publisher Date	2010-09-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Calcium-binding Proteins Chemistry Euplotes Fluorescence Resonance Energy Transfer Methods Protozoan Proteins Terbium Tyrosine Amino Acid Sequence Animals Calcium Metabolism Genetics Humans Models, Molecular Molecular Sequence Data Mutagenesis, Site-directed Protein Binding Sequence Alignment Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Critical role of tyrosine 79 in the fluorescence resonance energy transfer and terbium(III)-dependent self-assembly of ciliate Euplotes octocarinatus centrin

The biochemical effect of Ser166 phosphorylation on Euplotes octocarinatus centrin

The interaction between lanthanide (III) and N-terminal domain of Euplotes octocarinatus centrin.

The characterization for the binding of calcium and terbium to Euplotes octocarinatus centrin.

Analysis of the role of Mg²âº on conformational change and target recognition by ciliate Euplotes octocarinatus centrin.

Role of four conserved aspartic acid residues of EF-loops in the metal ion binding and in the self-assembly of ciliate Euplotes octocarinatus centrin

The binding sites of class I release factor (eRF1) toward class II release factor (eRF3) in Euplotes octocarinatus.

Electrochemical study of the interaction between Eu3+ and ciliate Euplotes octocarinatus centrin

Role of Asp37 in metal-binding and conformational change of ciliate Euplotes octocarinatus centrin

Critical role of tyrosine 79 in the fluorescence resonance energy transfer and terbium(III)-dependent self-assembly of ciliate Euplotes octocarinatus centrin.

Similar Documents

Critical role of tyrosine 79 in the fluorescence resonance energy transfer and terbium(III)-dependent self-assembly of ciliate Euplotes octocarinatus centrin

The biochemical effect of Ser166 phosphorylation on Euplotes octocarinatus centrin

The interaction between lanthanide (III) and N-terminal domain of Euplotes octocarinatus centrin.

The characterization for the binding of calcium and terbium to Euplotes octocarinatus centrin.

Analysis of the role of Mg²âº on conformational change and target recognition by ciliate Euplotes octocarinatus centrin.

Role of four conserved aspartic acid residues of EF-loops in the metal ion binding and in the self-assembly of ciliate Euplotes octocarinatus centrin

The binding sites of class I release factor (eRF1) toward class II release factor (eRF3) in Euplotes octocarinatus.

Electrochemical study of the interaction between Eu3+ and ciliate Euplotes octocarinatus centrin

Role of Asp37 in metal-binding and conformational change of ciliate Euplotes octocarinatus centrin

Critical role of tyrosine 79 in the fluorescence resonance energy transfer and terbium(III)-dependent self-assembly of ciliate Euplotes octocarinatus centrin.

Analysis of the role of Mg²âº on conformational change and target recognition by ciliate Euplotes octocarinatus centrin.