NDLI: Role of four conserved aspartic acid residues of EF-loops in the metal ion binding and in the self-assembly of ciliate Euplotes octocarinatus centrin

Please wait, while we are loading the content...

Expression of the zinc importer protein ZIP9/SLC39A9 in glioblastoma cells affects phosphorylation states of p53 and GSK-3β and causes increased cell migration

After oxidation, zinc nanoparticles lose their ability to enhance responses to odorants

The impact of lactoferrin with different levels of metal saturation on the intestinal epithelial barrier function and mucosal inflammation

Hypoxia-selective inhibition of angiogenesis development by NAMI-A analogues

Role of four conserved aspartic acid residues of EF-loops in the metal ion binding and in the self-assembly of ciliate Euplotes octocarinatus centrin

Melatonin restricts Pb-induced PCD by enhancing BI-1 expression in tobacco suspension cells

Intracellular uptake of an antitumor-active azole-bridged dinuclear platinum(II) complex in cisplatin-resistant tumor cells

A universal assay for the detection of siderophore activity in natural waters

A broad mercury resistant strain of Pseudomonas putida secretes pyoverdine under limited iron conditions and high mercury concentrations

BioMetals : Volume 29, Issue 5, October 2016

BioMetals : Volume 29, Issue 4, August 2016

BioMetals : Volume 29, Issue 3, June 2016

BioMetals : Volume 29, Issue 2, April 2016

BioMetals : Volume 29, Issue 1, February 2016

BioMetals : Volume 28

BioMetals : Volume 27

BioMetals : Volume 26

BioMetals : Volume 25

BioMetals : Volume 24

BioMetals : Volume 23

BioMetals : Volume 22

BioMetals : Volume 21

BioMetals : Volume 20

BioMetals : Volume 19

BioMetals : Volume 18

BioMetals : Volume 17

BioMetals : Volume 16

BioMetals : Volume 15

BioMetals : Volume 14

BioMetals : Volume 13

BioMetals : Volume 12

BioMetals : Volume 11

BioMetals : Volume 10

Role of four conserved aspartic acid residues of EF-loops in the metal ion binding and in the self-assembly of ciliate Euplotes octocarinatus centrin

Content Provider	Springer Nature Link
Author	Liu, Wen Duan, Lian Sun, Tijian Yang, Binsheng
Copyright Year	2016
Abstract	Ciliate Euplotes octocarinatus centrin (EoCen) is an EF-hand calcium-binding protein closely related to the prototypical calcium sensor protein calmodulin. Four mutants (D37K, D73K, D110K and D146K) were created firstly to elucidate the importance of the first aspartic acid residues (Asp37, Asp73, Asp110 and Asp146) in the beginning of the four EF-loops of EoCen. Aromatic-sensitized Tb3+ fluorescence indicates that the aspartic acid residues are very important for the metal-binding of EoCen, except for Asp73 (in EF-loop II). Resonance light scattering (RLS) measurements for different metal ions (Ca2+ and Tb3+) binding proteins suggest that the order of four conserved aspartic acid residues for contributing to the self-assembly of EoCen is Asp37 > Asp146 > Asp110 > Asp73. Cross-linking experiment also exhibits that Asp37 and Asp146 play critical role in the self-assembly of EoCen. Asp37, in site I, which is located in the N-terminal domain, plays the most important role in the metal ion-dependent self-assembly of EoCen, and there is cooperativity between N-terminal and C-terminal domain (especially the site IV). In addition, the dependence of Tb3+ induced self-assembly of EoCen and the mutants on various factors, including ionic strength and pH, were characterized using RLS. Finally, 2-p-toluidinylnaphthalene-6-sulfonate (TNS) binding, ionic strength and pH control experiments indicate that in the process of EoCen self-assembly, molecular interactions are mediated by both electrostatic and hydrophobic forces, and the hydrophobic interaction has the important status.
Starting Page	1047
Ending Page	1058
Page Count	12
File Format	PDF
ISSN	09660844
Journal	BioMetals
Volume Number	29
Issue Number	6
e-ISSN	15728773
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-10-14
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Centrin Aspartic acid Terbium(III) Self-assembly Biochemistry Pharmacology/Toxicology Microbiology Cell Biology Plant Physiology Medicine/Public Health
Content Type	Text
Resource Type	Article
Subject	Metals and Alloys Biochemistry, Genetics and Molecular Biology Biomaterials Agricultural and Biological Sciences

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Critical role of tyrosine 79 in the fluorescence resonance energy transfer and terbium(III)-dependent self-assembly of ciliate Euplotes octocarinatus centrin

Role of Asp37 in metal-binding and conformational change of ciliate Euplotes octocarinatus centrin

Metal ion-binding properties of wild-type and mutant D37K of ciliate Euplotes octocarinatus centrin

Critical role of tyrosine 79 in the fluorescence resonance energy transfer and terbium(III)-dependent self-assembly of ciliate Euplotes octocarinatus centrin.

The characterization for the binding of calcium and terbium to Euplotes octocarinatus centrin.

Spectral study on the interaction of ciliate Euplotes octocarinatus centrin and metal ions

Electrochemical study of the interaction between Eu3+ and ciliate Euplotes octocarinatus centrin

Crystal structures of the trimeric N-terminal domain of Ciliate Euplotes octocarinatus Centrin

Binding of Euplotes Octocarinatus centrin with target peptide melittin

Role of four conserved aspartic acid residues of EF-loops in the metal ion binding and in the self-assembly of ciliate Euplotes octocarinatus centrin

Similar Documents

Critical role of tyrosine 79 in the fluorescence resonance energy transfer and terbium(III)-dependent self-assembly of ciliate Euplotes octocarinatus centrin

Role of Asp37 in metal-binding and conformational change of ciliate Euplotes octocarinatus centrin

Metal ion-binding properties of wild-type and mutant D37K of ciliate Euplotes octocarinatus centrin

Critical role of tyrosine 79 in the fluorescence resonance energy transfer and terbium(III)-dependent self-assembly of ciliate Euplotes octocarinatus centrin.

The characterization for the binding of calcium and terbium to Euplotes octocarinatus centrin.

Spectral study on the interaction of ciliate Euplotes octocarinatus centrin and metal ions

Electrochemical study of the interaction between Eu3+ and ciliate Euplotes octocarinatus centrin

Crystal structures of the trimeric N-terminal domain of Ciliate Euplotes octocarinatus Centrin

Binding of Euplotes Octocarinatus centrin with target peptide melittin

Role of four conserved aspartic acid residues of EF-loops in the metal ion binding and in the self-assembly of ciliate Euplotes octocarinatus centrin