NDLI: Exploitation of a 'hockey-puck' phenotype to identify pilus and biofilm regulators in Serratia marcescens through genetic analysis.

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Exploitation of a 'hockey-puck' phenotype to identify pilus and biofilm regulators in Serratia marcescens through genetic analysis.

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Exploitation of a 'hockey-puck' phenotype to identify pilus and biofilm regulators in Serratia marcescens through genetic analysis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Shanks, Robert M. Q. Stella, Nicholas A. Brothers, Kimberly M. Polaski, Denise M.
Description	Country affiliation: United States Author Affiliation: Shanks RM ( The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Sciences Research Center, Eye and Ear Institute, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pa., USA.); Stella NA ( The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Sciences Research Center, Eye and Ear Institute, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pa., USA.); Brothers KM ( The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Sciences Research Center, Eye and Ear Institute, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pa., USA.); Polaski DM ( The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Sciences Research Center, Eye and Ear Institute, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pa., USA.)
Abstract	Pili are essential adhesive determinants for many bacterial pathogens. A suppressor mutation screen that takes advantage of a pilus-mediated self-aggregative 'hockey-puck' colony phenotype was designed to identify novel regulators of type I pili in Serratia marcescens. Mutations that decreased pilus biosynthesis mapped to the fimABCD operon; to the genes alaT, fkpA, and oxyR; upstream of the flagellar master regulator operon flhDC; and to an uncharacterized gene encoding a predicted DUF1401 domain. Biofilm formation and pilus-dependent agglutination assays were used to characterize the relative importance of the identified genes in pilus biosynthesis. Additional mutagenic or complementation analysis was used to verify the role of candidate genes in pilus biosynthesis. Presented data support a model that CRP negatively regulates pilus biosynthesis through increased expression of flhDC and decreased expression of oxyR. Further studies are warranted to determine the mechanism by which these genes mediate pilus biosynthesis or function.
File Format	HTM / HTML
ISSN	00084166
e-ISSN	14803275
Journal	Canadian Journal of Microbiology
Issue Number	1
Volume Number	62
Language	English
Publisher	Canadian Science Publishing
Publisher Date	2016-01-01
Publisher Place	Canada
Access Restriction	Open
Subject Keyword	Discipline Microbiology Biofilms Fimbriae, Bacterial Physiology Serratia Marcescens Bacterial Adhesion Operon Phenotype Genetics Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology Immunology Microbiology Applied Microbiology and Biotechnology

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