NDLI: Mutation of crp mediates Serratia marcescens serralysin and global secreted protein production.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Shanks, Robert M. Q. Stella, Nicholas A. Arena, Kristin E. Fender, James E.
Description	Country affiliation: United States Author Affiliation: Shanks RM ( The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Sciences Research Center, Eye and Ear Institute, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15208, USA. shanksrm@upmc.edu)
Abstract	The bacterial species Serratia marcescens secretes both beneficial and cytotoxic proteins. Here we report that a crp mutant exhibited elevated secreted protease activity. A genetic screen revealed that the gene coding for the metalloprotease serralysin was necessary for the elevated proteolysis, and this was confirmed by western blot analysis. Proteomic analysis of secreted proteins corroborated increased secretion of serralysin protease by crp mutants compared to the wild type. The crp-mutant-secreted fractions also contained less chitinase and chitin binding protein. These data support the hypothesis that cAMP-CRP is an upstream indirect regulator of serralysin production and they provide novel insight into the S. marcescens secretome.
File Format	HTM / HTML
ISSN	09232508
e-ISSN	17697123
DOI	10.1016/j.resmic.2012.10.006
Journal	Research in Microbiology
Issue Number	1
Volume Number	164
Language	English
Publisher	Elsevier
Publisher Date	2013-01-01
Publisher Place	France
Access Restriction	Open
Subject Keyword	Discipline Microbiology Bacterial Proteins Genetics Metabolism Mutation Serratia Marcescens Cyclic Amp Enzyme Activation Lipase Peptide Hydrolases Proteome Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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Mutation of crp mediates Serratia marcescens serralysin and global secreted protein production

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