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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Li, Yuce Tian, Huayu Ding, Jianxun Lin, Lin Chen, Jie Gao, Shiqian Chen, Xuesi |
| Description | Author Affiliation: Li Y ( Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.); Tian H ( University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.); Ding J ( Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.); Lin L ( Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.); Chen J ( Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.); Gao S ( Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.); Chen X ( Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.) |
| Abstract | RNA interference (RNAi) provides the promising treatments of gene-related diseases while hindered by the lack of highly efficient delivery platform with low cytotoxicity. Moreover, the intracellular fates of nonviral gene carriers are closely related to their internalization pathway, and eventually influence their RNAi efficiency. Herein, a series of guanidinated thiourea-modified polyethylenimines (PEI-MTU-Gs) are synthesized and utilized as the efficient carriers of small interfering RNA (siRNA) with up to 71.6% inhibition of luciferase activity in the luciferase-expressing cell lines (i.e., HeLa/Luc cells). The introduction of noncationic hydrogen bond donors, that is, thiourea groups, provides the carriers with much lower cytotoxicities and relatively looser complex structures that facilitate the intracellular release of siRNAs. Furthermore, the multiguanidino structures endow the PEI-MTU-G/siRNA complexes with the ability to directly penetrate cell membrane, which facilitates the cellular internalization while avoiding them suffering from the rigorous lysosomes. The results demonstrate PEI-MTU35 -Gs as promising siRNA carriers for further gene therapy. |
| File Format | HTM / HTML |
| ISSN | 21922640 |
| Issue Number | 9 |
| Volume Number | 4 |
| e-ISSN | 21922659 |
| Journal | Advanced Healthcare Materials |
| Language | English |
| Publisher | Wiley-VCH |
| Publisher Date | 2015-06-24 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biomedical Engineering Cell Membrane Metabolism Drug Delivery Systems Methods Guanidine Polyethyleneimine Rna, Small Interfering Thiourea Gene Expression Regulation Drug Effects Chemistry Pharmacology Hela Cells Humans Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biomaterials Biomedical Engineering Pharmaceutical Science |
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