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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Millan, Christopher Cavalli, Emma Groth, Thomas Maniura-Weber, Katharina Zenobi-Wong, Marcy |
| Description | Country affiliation: Switzerland Author Affiliation: Millan C ( Cartilage Engineering + Regeneration Laboratory, ETH Zürich, Otto-Stern-Weg 7, 8093, Zürich, Switzerland.); Cavalli E ( Cartilage Engineering + Regeneration Laboratory, ETH Zürich, Otto-Stern-Weg 7, 8093, Zürich, Switzerland.); Groth T ( Biomedical Materials Group, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120, Halle (Saale), Germany.); Maniura-Weber K ( Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014, St. Gallen, Switzerland.); Zenobi-Wong M ( Cartilage Engineering + Regeneration Laboratory, ETH Zürich, Otto-Stern-Weg 7, 8093, Zürich, Switzerland.) |
| Abstract | A universal method for reproducibly directing stem cell differentiation remains a major challenge for clinical applications involving cell-based therapies. The standard approach for chondrogenic induction by micromass pellet culture is highly susceptible to interdonor variability. A novel method for the fabrication of condensation-like engineered microtissues (EMTs) that utilizes hydrophilic polysaccharides to induce cell aggregation is reported here. Chondrogenesis of mesenchymal stem cells (MSCs) in EMTs is significantly enhanced compared to micromass pellets made by centrifugation measured by type II collagen gene expression, dimethylmethylene blue assay, and histology. MSCs from aged donors that fail to differentiate in pellet culture are successfully induced to synthesize cartilage-specific matrix in EMTs under identical media conditions. Furthermore, the EMT polysaccharides support the loading and release of the chondroinduction factor transforming growth factor ß3 (TGF-ß3). TGF-ß-loaded EMTs (EMT(+TGF) ) facilitate cartilaginous tissue formation during culture in media not supplemented with the growth factor. The clinical potential of this approach is demonstrated in an explant defect model where EMT(+TGF) from aged MSCs synthesize de novo tissue containing sulfated glycosaminoglycans and type II collagen in situ. |
| File Format | HTM / HTML |
| ISSN | 21922640 |
| Issue Number | 9 |
| Volume Number | 4 |
| e-ISSN | 21922659 |
| Journal | Advanced Healthcare Materials |
| Language | English |
| Publisher | Wiley-VCH |
| Publisher Date | 2015-06-24 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biomedical Engineering Aging Metabolism Cartilage Cell Differentiation Chondrogenesis Mesenchymal Stromal Cells Tissue Engineering Animals Cytology Cattle Cells, Cultured Humans Schiff Bases Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biomaterials Biomedical Engineering Pharmaceutical Science |
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