| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Lee, Hyeong Jin Kim, Yong Bok Ahn, Seung Hyun Lee, Ji-Seon Jang, Chul Ho Yoon, Hyeon Chun, Wook Kim, Geun Hyung |
| Description |
Country affiliation: South Korea Author Affiliation: Lee HJ ( Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, South Korea.); Kim YB ( Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, South Korea.); Ahn SH ( Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, South Korea.); Lee JS ( Department of Surgery, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, South Korea.); Jang CH ( Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea.); Yoon H ( Department of Surgery, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, South Korea.); Chun W ( Department of Surgery, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, South Korea.); Kim GH ( Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, South Korea.) |
| Abstract |
Cell-printing methods have been used widely in tissue regeneration because they enable fabricating biomimetic 3D structures laden with various cells. To achieve a cell-matrix block, various natural hydrogels that are nontoxic, biocompatible, and printable have been combined to obtain 'bioinks.' Unfortunately, most bioinks, including those with alginates, show low cell-activating properties. Here, a strategy for obtaining highly bioactive ink, which consisted of collagen/extracellular matrix (ECM) and alginate, for printing 3D porous cell blocks is developed. An in vitro assessment of the 3D porous structures laden with preosteoblasts and human adipose stem cells (hASCs) demonstrates that the cells in the bioinks are viable. Osteogenic activities with the designed bioinks show much higher levels than with the 'conventional' alginate-based bioink. Furthermore, the hepatogenic differentiation ability of hASCs with the bioink is evaluated using the liver-specific genes, albumin, and TDO2, under hepatogenic differentiation conditions. The genes are activated within the 3D cell block fabricated using the new bioink. These results demonstrate that the 3D cell-laden structure fabricated using collagen/ECM-based bioinks can provide a novel platform for various tissue engineering applications. |
| File Format |
HTM / HTML |
| ISSN |
21922640 |
| Issue Number |
9 |
| Volume Number |
4 |
| e-ISSN |
21922659 |
| Journal |
Advanced Healthcare Materials |
| Language |
English |
| Publisher |
Wiley-VCH |
| Publisher Date |
2015-06-24 |
| Publisher Place |
Germany |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Biomedical Engineering Adipose Tissue Metabolism Collagen Chemistry Extracellular Matrix Osteogenesis Stem Cells Tissue Scaffolds Cytology Alginates Cells, Cultured Glucuronic Acid Hexuronic Acids Humans Hydrogels Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Biomaterials Biomedical Engineering Pharmaceutical Science |
