NDLI: Matching X-ray beam and detector properties to protein crystals of different perfection.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Nave, Colin
Description	Country affiliation: United kingdom Author Affiliation: Nave C ( Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK.)
Abstract	An analysis is given of the effect of different beam and detector parameters on the sharpness of recorded diffraction features for macromolecular crystals of different quality. The crystal quality parameters include crystal strain, crystal or mosaic block size and mosaic block misorientation. Calculations are given for instrument parameters such as angular resolution of the detector, beam divergence and wavelength bandpass to be matched to the intrinsic diffraction properties from these crystals with the aim of obtaining the best possible data out of each crystal. Examples are given using typical crystal imperfections obtained from the literature for both room-temperature and cryo-cooled crystals. Possible implications for the choice of X-ray source, beamline design, detector specifications, instrument set-up and data processing are discussed, together with the limitations of the approach.
File Format	HTM / HTML
ISSN	09090495
e-ISSN	16005775
DOI	10.1107/S1600577514003609
Journal	Journal of Synchrotron Radiation
Issue Number	Pt 3
Volume Number	21
Language	English
Publisher	IUCr
Publisher Date	2014-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Radiology Crystallography, X-ray Models, Chemical Models, Molecular Proteins Chemistry Ultrastructure Computer Simulation Crystallization Equipment Design Equipment Failure Analysis Protein Conformation Reproducibility Of Results Scattering, Radiation Sensitivity And Specificity X-rays
Content Type	Text
Resource Type	Article
Subject	Nuclear and High Energy Physics Instrumentation Radiation

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