NDLI: Ultrastructural changes in the rat kidney after single dose of cyclophosphamide--possible roles for peroxisome proliferation and lysosomal dysfunction in cyclophosphamide-induced renal damage.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Abraham, Premila Isaac, Bina
Description	Country affiliation: India Author Affiliation: Abraham P ( Department of Biochemistry, Christian Medical College, Vellore, Tamil Nadu, India. premilaabraham@yahoo.com)
Abstract	Electron microscopy was used to examine changes in the subcellular organelles of the rat kidney at different time intervals after a single exposure to cyclophosphamide (CP). The morphological changes were studied at different time points (6 hrs, 16 hrs and 24 hrs) after a single-dose administration of CP. Six rats were killed at each time intervals after the administration of CP. Saline-treated rats served as controls. CP administration resulted in alterations in various subcellular organelles including peroxisomes, lysosomes, mitochondria, and the endoplasmic reticulum (ER) of the renal tubular epithelium as well as damage to the glomerulus. The basement membrane of the glomerulus was thickened. Many podocytes were destroyed. The nucleoplasm of the endothelial cell showed fewer granularities. The tubules were distorted and the brush border was destroyed. Two striking features in the renal tubular cells are increase in number and size of the peroxisomes (peroxisome proliferation) and decrease in the number of lysosomes. The mitochondria were elongated and the number was increased in the tubules of CP-treated rats. The ER was dilated. Cell necrosis was also seen. This study is an evidence of changes in morphology of rat kidney after induction of renal damage by a single dose of CP. Since transmission electron microscopy is the highest magnification tool at present, it can be useful in estimating the degree of injury and outcome of alternative treatment strategies in the management of CP-induced renal damage after establishing a scoring system.
File Format	HTM / HTML
ISSN	09603271
Issue Number	12
Volume Number	30
e-ISSN	14770903
Journal	Human & Experimental Toxicology
Language	English
Publisher	Sage Publication
Publisher Date	2011-12-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Toxicology Antineoplastic Agents, Alkylating Toxicity Cyclophosphamide Kidney Diseases Chemically Induced Kidney Drug Effects Lysosomes Peroxisomes Animals Pathology Kidney Glomerulus Ultrastructure Kidney Tubules Male Microscopy, Electron, Transmission Necrosis Organelles Rats Rats, Wistar Journal Article
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Medicine Toxicology

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