NDLI: Protective effect of Glycine max and Chrysanthemum indicum extracts against cisplatin-induced renal epithelial cell death.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pongjit, Kanittha Ninsontia, Chaunpit Chaotham, Chatchai Chanvorachote, Pithi
Description	Country affiliation: Thailand Author Affiliation: Pongjit K ( Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.)
Abstract	Although cisplatin is one of the most efficient chemotherapeutic agents for the treatment of solid tumors, frequently observed nephrotoxicity has limited its use in several patients. Materials and methods: The protective effect of Glycine max (GM) and Chrysanthemum indicum (CM) extracts on cisplatin-induced apoptosis in human proximal tubular HK-2 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Hoechst 33342, and propidium iodide assays. Reactive oxygen species (ROS) were determined by flow cytometry with 2,7-dichlorofluorescein diacetate $(DCFH_{2}-DA).$ Results: Cisplatin-induced renal cell toxicity through the induction of hydrogen peroxide $(H_{2}O_{2}^{•−})$ and hydroxyl radical (OH). CM extract protected cisplatin-induced apoptosis by its anti-oxidant activity against $H_{2}O_{2}$ and $OH^{•−},$ while GM extract scavenged only $H_{2}O_{2}.$ Furthermore, GM and CM extracts protect renal cells without significant interfering effect on cisplatin toxicity in lung cancer H460 and melanoma G361 cells. Conclusion: GM and CM extracts exhibited a promising protective effect on cisplatin-induced nephrotoxicity which could benefit the development for nephroprotective approaches.
File Format	HTM / HTML
ISSN	09603271
Issue Number	12
Volume Number	30
e-ISSN	14770903
Journal	Human & Experimental Toxicology
Language	English
Publisher	Sage Publication
Publisher Date	2011-12-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Toxicology Antineoplastic Agents Toxicity Cell Death Drug Effects Cisplatin Kidney Plant Extracts Pharmacology Protective Agents Apoptosis Cell Line, Tumor Cell Survival Chrysanthemum Chemistry Epithelium Metabolism Pathology Humans Kidney Tubules, Proximal Necrosis Chemically Induced Reactive Oxygen Species Soybeans Journal Article
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Medicine Toxicology

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