NDLI: Calpain-10 is a component of the obesity-related quantitative trait locus Adip1

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cheverud, James M. Fawcett, Gloria L. Jarvis, Joseph P. Norgard, Elizabeth A. Pavlicev, Mihaela Pletscher, L. Susan Polonsky, Kenneth S. Ye, Honggang Bell, Graeme I. Semenkovich, Clay F.
Description	Country affiliation: United States Author Affiliation: Cheverud JM ( Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO, USA. cheverud@pcg.wustl.edu)
Abstract	We previously mapped Adip1, an obesity quantitative trait locus (QTL), to the central portion of murine chromosome 1 containing the calpain-10 (Capn10) gene. Human studies have associated calpain-10 (CAPN10) variants with type 2 diabetes and various metabolic traits. We performed a quantitative hybrid complementation test (QHCT) to determine whether differences attributed to Adip1 are the result of variant Capn10 alleles in LG/J and SM/J mice. We crossed LG/J and SM/J to wild-type (C57BL/6J) and Capn10 knockout (Capn10(-/-)) mice to form four F(1) hybrid groups: LG/J by wild-type, LG/J by Capn10(-/-), SM/J by wild-type, and SM/J by Capn10(-/-). We performed a two-way ANOVA with the experimental strain, tester strain, and their interaction as the factors. Significant interaction indicates a quantitative failure to complement. We found failure to complement for fat, organ, and body weights, and leptin, female free fatty acid, and triglyceride levels. Capn10(-/-) resulted in heavier weights and higher serum levels in LG/J crosses but not in SM/J crosses. For glucose tolerance and insulin response tests, the Capn10(-/-) allele resulted in lower glucose levels in crosses with SM/J but had no effect in the LG/J crosses. Differences between the LG/J and SM/J Capn10 alleles are the likely source of some of the QTL effects mapped to Adip1 in the LG/J-by-SM/J cross. Capn10 plays an important role in regulating obesity and diabetes in mice.
File Format	HTM / HTML
ISSN	00222275
e-ISSN	15397262
DOI	10.1194/jlr.M900128
Journal	The Journal of Lipid Research
Issue Number	5
Volume Number	51
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2010-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biochemistry Calpain Genetics Obesity Quantitative Trait Loci Alleles Animals Deficiency Metabolism Gene Knockout Techniques Genetic Complementation Test Genetic Variation Hybridization, Genetic Mice Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Endocrinology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Calpain-10 is a component of the obesity-related quantitative trait locus Adip1

Fine-mapping of Obesity-related Quantitative Trait Loci in an $F_{9/10}$ Advanced Intercross Line

Cis-acting expression quantitative trait loci in mice.

Defining the functional boundaries of the Gata2 locus by rescue with a linked bacterial artificial chromosome transgene.

Genetic basis of HDL variation in 129/SvImJ and C57BL/6J mice: importance of testing candidate genes in targeted mutant mice

Complementation testing identifies genes mediating effects at quantitative trait loci underlying fear-related behavior.

Complementation test of Rpe65 knockout and tvrm148.

Genetic, epigenetic, and gene-by-diet interaction effects underlie variation in serum lipids in a LG/JxSM/J murine model.

A quantitative trait locus for variation in dopamine metabolism mapped in a primate model using reference sequences from related species.

Calpain-10 is a component of the obesity-related quantitative trait locus Adip1

Similar Documents

Calpain-10 is a component of the obesity-related quantitative trait locus Adip1

Fine-mapping of Obesity-related Quantitative Trait Loci in an $F_{9/10}$ Advanced Intercross Line

Cis-acting expression quantitative trait loci in mice.

Defining the functional boundaries of the Gata2 locus by rescue with a linked bacterial artificial chromosome transgene.

Genetic basis of HDL variation in 129/SvImJ and C57BL/6J mice: importance of testing candidate genes in targeted mutant mice

Complementation testing identifies genes mediating effects at quantitative trait loci underlying fear-related behavior.

Complementation test of Rpe65 knockout and tvrm148.

Genetic, epigenetic, and gene-by-diet interaction effects underlie variation in serum lipids in a LG/JxSM/J murine model.

A quantitative trait locus for variation in dopamine metabolism mapped in a primate model using reference sequences from related species.

Calpain-10 is a component of the obesity-related quantitative trait locus Adip1