NDLI: Single embryo and oocyte lipid fingerprinting by mass spectrometry.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ferreira, Christina R. Saraiva, Sergio A. Catharino, Rodrigo R. Garcia, Jerusa S. Gozzo, Fabio C. Sanvido, Gustavo B. Santos, Luiz Fernando A. Lo Turco, Edson G. Pontes, José Henrique F. Basso, Andréa C. Bertolla, Ricardo P. Sartori, Roberto Guardieiro, Monique M. Perecin, Felipe Meirelles, Flávio V. Sangalli, Juliano R. Eberlin, Marcos N.
Description	Country affiliation: Brazil Author Affiliation: Ferreira CR ( Thomson Mass Spectrometry Laboratory, University of Campinas, Campinas, SP, Brazil. christina@iqm.unicamp.br)
Abstract	Methods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of single and intact embryos or oocytes from various species. Biological samples were simply moisturized with the matrix solution and characteristic lipid (represented by phosphatidylcholines, sphingomyelins and triacylglycerols) profiles were obtained via MALDI-MS. As representative examples, human, bovine, sheep and fish oocytes, as well as bovine and insect embryos were analyzed. MALDI-MS is shown to be capable of providing characteristic lipid profiles of gametes and embryos and also to respond to modifications due to developmental stages and in vitro culture conditions of bovine embryos. Investigation in developmental biology of the biological roles of structural and reserve lipids in embryos and oocytes should therefore benefit from these rapid MALDI-MS profiles from single and intact species.
File Format	HTM / HTML
ISSN	00222275
e-ISSN	15397262
DOI	10.1194/jlr.D001768
Journal	The Journal of Lipid Research
Issue Number	5
Volume Number	51
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2010-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biochemistry Embryo, Mammalian Chemistry Lipids Oocytes Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Animals Embryology Embryonic Development Species Specificity Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Endocrinology

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