NDLI: Caveolins sequester FA on the cytoplasmic leaflet of the plasma membrane, augment triglyceride formation, and protect cells from lipotoxicity.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Simard, Jeffrey R. Meshulam, Tova Pillai, Biju K. Kirber, Michael T. Brunaldi, Kellen Xu, Su Pilch, Paul F. Hamilton, James A.
Description	Country affiliation: United States Author Affiliation: Simard JR ( Department of Biophysics and Physiology, Boston University School of Medicine, Boston, MA, USA.)
Abstract	Ectopic expression of caveolin-1 in HEK293 cells enhances FA sequestration in membranes as measured by a pH-sensitive fluorescent dye (1). We hypothesized that sequestration of FA is due to the enrichment of caveolin in the cytosolic leaflet and its ability to facilitate the formation of lipid rafts to buffer high FA levels. Here we show that ec-topic expression of caveolin-3 also results in enhanced FA sequestration. To further discriminate the effect that caveolins have on transmembrane FA movement and distribution, we labeled the outer membrane leaflet with fluorescein-phosphatidylethanolamine (FPE), whose emission is quenched by the presence of FA anions. Real-time measurements made with FPE and control experiments with positively charged fatty amines support our hypothesis that caveolins promote localization of FA anions through interactions with basic amino acid residues (lysines and arginines) present at the C termini of caveolins-1 and -3.
File Format	HTM / HTML
ISSN	00222275
e-ISSN	15397262
DOI	10.1194/jlr.M900251
Journal	The Journal of Lipid Research
Issue Number	5
Volume Number	51
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2010-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biochemistry Caveolins Metabolism Cell Membrane Cytoplasm Fatty Acids Toxicity Triglycerides Biosynthesis Amines Chemistry Caveolin 1 Caveolin 3 Cell Line Dose-response Relationship, Drug Extracellular Space Fluoresceins Gene Expression Regulation Movement Phosphatidylethanolamines Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Endocrinology

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