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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ferreira, Mônica S. de Oliveira, Diogo N. Gonçalves, Roseli F. Catharino, Rodrigo R. |
| Description | Country affiliation: Brazil Author Affiliation: Ferreira MS ( Innovare Biomarkers Laboratory, Medicine and Experimental Surgery Nucleus, University of Campinas, Campinas 13083-877, SP, Brazil.); de Oliveira DN ( Innovare Biomarkers Laboratory, Medicine and Experimental Surgery Nucleus, University of Campinas, Campinas 13083-877, SP, Brazil.); Gonçalves RF ( Department of Animal Reproduction, Veterinary Medicine College of São Paulo University, São Paulo 05508-270, SP, Brazil.); Catharino RR ( Innovare Biomarkers Laboratory, Medicine and Experimental Surgery Nucleus, University of Campinas, Campinas 13083-877, SP, Brazil. Electronic address: rrc@fcm.unicamp.br.) |
| Abstract | Lipid pathways play important biological roles in mammalian embryology, directing early developmental pathways to differentiation. Phospholipids and triglycerides, among others, are the main composing lipids of zona pellucida in several embryo species. Lipid analysis in embryos by mass spectrometry usually requires sample preparation and/or matrix application. This novel approach using silica plate laser desorption/ionization mass spectrometry imaging (SP-LDI-MSI) allows direct single-cell imaging and embryo region discrimination with no matrix coating. Its application is herein described for two- and eight-cell embryos. Lipid biomarkers for blastomere and intact zona pellucida are reported and corroborated by both fragmentation reactions (MS/MS) and images. Results obtained in this work are understood to be of great use for further developments on in vitro bovine fertilization. Since much of the processes can be monitored by characteristic biomarkers, it is now possible to precisely identify cell division errors during early embryo stages, as well as evaluate pre-implantation conditions. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 807 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-01-07 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Embryo, Mammalian Metabolism Lipids Analysis Silicon Dioxide Chemistry Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Animals Biological Markers Cattle Principal Component Analysis Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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