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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Venken, Koen J. T. Schulze, Karen L. Haelterman, Nele A. Pan, Hongling He, Yuchun Evans-Holm, Martha Carlson, Joseph W. Levis, Robert W. Spradling, Allan C. Hoskins, Roger A. Bellen, Hugo J. |
| Description | Country affiliation: United States Author Affiliation: Venken KJ ( Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA. kv134369@bcm.edu) |
| Abstract | We demonstrate the versatility of a collection of insertions of the transposon Minos-mediated integration cassette (MiMIC), in Drosophila melanogaster. MiMIC contains a gene-trap cassette and the yellow+ marker flanked by two inverted bacteriophage ΦC31 integrase attP sites. MiMIC integrates almost at random in the genome to create sites for DNAmanipulation. The attP sites allow the replacement of the intervening sequence of the transposon with any other sequence through recombinase-mediated cassette exchange (RMCE). We can revert insertions that function as gene traps and cause mutant phenotypes to revert to wild type by RMCE and modify insertions to control GAL4 or QF overexpression systems or perform lineage analysis using the Flp recombinase system. Insertions in coding introns can be exchanged with protein-tag cassettes to create fusion proteins to follow protein expression and perform biochemical experiments. The applications of MiMIC vastly extend the D. melanogaster toolkit. |
| File Format | HTM / HTML |
| ISSN | 15487091 |
| e-ISSN | 15487105 |
| Journal | Nature Methods |
| Issue Number | 9 |
| Volume Number | 8 |
| Language | English |
| Publisher | Nature Publishing Group |
| Publisher Date | 2011-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Clinical Laboratory Techniques Dna Transposable Elements Genetics Drosophila Melanogaster Animals Bioengineering Drosophila Proteins Gene Expression Regulation Introns Mutagenesis, Insertional Recombinant Fusion Proteins Repetitive Sequences, Nucleic Acid Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology Biotechnology |
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