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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Subach, Oksana M. Patterson, George H. Ting, Li-Min Wang, Yarong Condeelis, John S. Verkhusha, Vladislav V. |
| Description | Country affiliation: United States Author Affiliation: Subach OM ( Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, USA.) |
| Abstract | We report a photoswitchable monomeric Orange (PSmOrange) protein that is initially orange (excitation, 548 nm; emission, 565 nm) but becomes far-red (excitation, 636 nm; emission, 662 nm) after irradiation with blue-green light. Compared to its parental orange proteins, PSmOrange has greater brightness, faster maturation, higher photoconversion contrast and better photostability. The red-shifted spectra of both forms of PSmOrange enable its simultaneous use with cyan-to-green photoswitchable proteins to study four intracellular populations. Photoconverted PSmOrange has, to our knowledge, the most far-red excitation peak of all GFP-like fluorescent proteins, provides diffraction-limited and super-resolution imaging in the far-red light range, is optimally excited with common red lasers, and can be photoconverted subcutaneously in a mouse. PSmOrange photoswitching occurs via a two-step photo-oxidation process, which causes cleavage of the polypeptide backbone. The far-red fluorescence of photoconverted PSmOrange results from a new chromophore containing N-acylimine with a co-planar carbon-oxygen double bond. |
| File Format | HTM / HTML |
| ISSN | 15487091 |
| e-ISSN | 15487105 |
| DOI | 10.1038/nmeth.1664 |
| Journal | Nature Methods |
| Issue Number | 9 |
| Volume Number | 8 |
| Language | English |
| Publisher | Nature Publishing Group |
| Publisher Date | 2011-07-31 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Clinical Laboratory Techniques Luminescent Proteins Chemistry Amino Acid Sequence Animals Cos Cells Cercopithecus Aethiops Fluorescence Fluorescent Dyes Radiation Effects Hek293 Cells Hela Cells Mice Molecular Sequence Data |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology Biotechnology |
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